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中国农学通报 ›› 2014, Vol. 30 ›› Issue (12): 160-164.doi: 10.11924/j.issn.1000-6850.2013-2025

所属专题: 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

4种大戟科作物嫩茎若干生理生化指标的比较

谭德冠 邹发贵 付莉莉 韩冰莹 孙雪飘 梁魁格 张家明   

  • 收稿日期:2013-07-25 修回日期:2013-09-11 出版日期:2014-04-25 发布日期:2014-04-25
  • 基金资助:
    973项目“国家重要热带作物木薯新品种选育的基础研究”(2010CB126600);国家自然科学基金资助“巴西橡胶树愈伤组织乳管细胞发育机制的研究”(31070637);中国热带农业科学院中央级公益性科研院所基本科研业务费专项资金资助“橡胶树内生生物的分离、鉴定和开发利用”(ITBB110307);海南省重大科技项目子课题“热带生物种质与基因资源研究”(ZDZX2013023-1)。

Comparison of Some Crucial Physiological and Biochemical Parameters of Young Stems in Four Crops of Euphorbiaceae

  • Received:2013-07-25 Revised:2013-09-11 Online:2014-04-25 Published:2014-04-25

摘要: 研究4种扦插能力差异较大的大戟科作物嫩茎碳水化合物、蛋白质等一些重要的生理生化指标,对探明大戟科作物的扦插机理具有重要意义。采用苯酚法测定嫩茎可溶性糖、淀粉含量,采用考马斯亮法测定嫩茎可溶性蛋白含量,采用石蜡制片、高碘酸-锡夫染色法检测各种嫩茎淀粉的分布情况。结果表明,木薯品种‘华南5号’(易扦插)、小桐子品系‘无系性1号’(易扦插)、木薯野生近缘种‘WH14’(难扦插)嫩茎可溶性糖含量较高,同时前两者淀粉含量也较高,而橡胶树品种‘热研7-33-97’(难扦插)嫩茎可溶性糖、淀粉含量最低。‘WH14’、‘华南5号’嫩茎的可溶性蛋白含量最高,而‘无性系1号’、‘热研7- 33-97’最低。高碘酸-锡夫染色发现4种作物嫩茎的淀粉分布有较大的差异,其中‘无性系1号’嫩茎皮层、韧皮部贮藏大量的淀粉,‘华南5号’初生木质部贮藏大量淀粉,而‘热研7-33-97’、‘WH14’嫩茎只在韧皮部中含有极少量淀粉。上述结果说明大戟科作物嫩茎的高含量淀粉在扦插生根中起重要作用,而其蛋白质含量与扦插生根难易程度不相关。

关键词: 鱼类, 鱼类

Abstract: Some crucial physiological and biochemical parameters including the carbohydrate and protein in the young stems of four crops of Euphorbiaceae with different cuttage ability were studied in this paper, which had great significance to explore the cuttage mechanism of Euphorbiaceae. The soluble sugar and starch content was analyzed by phenol method, the soluble protein content was examined by Bradford method, and starch distribution of young stems was investigated by paraffin section and periodic acid-schiff staining. The results showed that young stems of‘SC5’(Manihot esculenta crantz) (with a high cuttage ability),‘Clone1’(Jatropha curcas) (with a high cuttage ability), and‘WH14’(Manihot esculenta sub. spp. flabellifolia) (with a poor cuttage ability) had high concentration of soluble sugar, and that of‘SC5’and‘Clone1’had high concentration of starch, but that of‘Reyan7-33-97’(Hevea brasiliensis) (with a poor cuttage ability) had the lowest concentration of soluble sugar and starch in them. Young stems of‘WH14’and‘SC5’contained significantly higher concentration of soluble protein than that of‘Clone1’and‘Reyan7- 33- 97’. Periodic acid- schiff staining revealed that the starch in young stems of these four crops distributed differently. The cortex and phloem of‘Clone1’and the primary xylem of‘SC5’stored much starch in their young stem. However,‘Reyan7-33-97’and‘WH14’only stored a little starch in the phloem. These results suggested that high starch content in young stems of Euphorbiaceae might be essential in cuttage of Euphorbiaceae, and protein concentration in cutting stems of Euphorbiaceae might be not related to cuttage.