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中国农学通报 ›› 2014, Vol. 30 ›› Issue (13): 107-111.doi: 10.11924/j.issn.1000-6850.2013-2641

所属专题: 生物技术 园艺

• 林学 园艺 园林 • 上一篇    下一篇

青花菜生育期的主基因+多基因遗传分析

何道根 张志仙 朱长志   

  • 收稿日期:2013-10-11 修回日期:2013-11-25 出版日期:2014-05-05 发布日期:2014-05-05
  • 基金资助:
    浙江省农业新品种选育重大科技专项项目“青花菜替代新品种选育”(2012C12903-3-2);浙江省产业技术创新战略联盟项目“青花菜新品种选育”(瓜菜联盟2010-04);台州市科技计划项目“青花菜胞质雄性不育系选育及应用”(102KY01);台州市农业类重点科技项目“西兰花种质资源创新及应用”(121zd13)。

Genetic Analysis of Growth Period Using Mixed Major Gene Plus Polygene Inheritance Model in Brassica oleracea L. var. italica

  • Received:2013-10-11 Revised:2013-11-25 Online:2014-05-05 Published:2014-05-05

摘要: 为了解青花菜生育期的遗传机理及新品种选育提供理论指导,以定植至现蕾的天数作为青花菜生育期的参考指标,以2个生育期差异较大的青花菜自交系作亲本配制的6个联合世代(P1、P2、F1、B1、B2和F2)群体为试验材料,采用植物数量性状主基因+多基因混合遗传模型分析方法对青花菜生育期进行遗传分析。结果表明:青花菜杂交种F1的生育期介于双亲之间,偏向于高亲值;生育期性状的遗传符合两对主基因加性-显性-上位性模型(B-1模型);B1、B2和F2世代主基因遗传率分别为44%、59%和56%,主基因遗传率中等,受环境的影响比较大,可以在早期世代进行初选。

关键词: 荧光定量PCR, 荧光定量PCR

Abstract: In order to analyze the genetic mechanism of growth period and provide the theoretical supports for breeding in broccoli (Brassica oleracea L. var. Italica), six generations (P1, P2, F1, B1, B2 and F2) derived from two morphologically dissimilar broccoli inbred lines were used for genetic analysis of growth period trait based on mixed major gene plus polygene inheritance model. We designated the growth period as the days from field planting to flower buds emergence in this experiment. The results showed that the mean values of F1 lied between two parents but it was much closer to the high valued parent in growth period traits. Meanwhile, the growth period traits were controlled by two additive-dominant-epitasis major genes (B- 1 Model). The major gene heritabilities of B1, B2 and F2 populations were 44%, 59% and 56%, respectively. And the major gene heritability was relatively moderate and was influenced by environment significantly, which could be used in the selection of early generation in broccoli.