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中国农学通报 ›› 2014, Vol. 30 ›› Issue (13): 137-141.doi: 10.11924/j.issn.1000-6850.2013-2765

所属专题: 园艺

• 林学 园艺 园林 • 上一篇    下一篇

基于AFLP技术的枣树主栽品种 亲缘关系的研究

谢永波 赵进红 王玉山 王利   

  • 收稿日期:2013-10-23 修回日期:2013-11-28 出版日期:2014-05-05 发布日期:2014-05-05
  • 基金资助:
    山东省良种工程项目 “泰安地方名产 “宁阳圆铃枣” 抗病品种选育与示范” (鲁科农字[2012]213号)。

Studies on Relationship ofMain Chinese jujube Cultivars by AFLP Markers

  • Received:2013-10-23 Revised:2013-11-28 Online:2014-05-05 Published:2014-05-05

摘要: 试验取30个枣树优良主栽品种进行AFLP分子标记,并进行遗传聚类分析,以期获得不同枣树品种之间的亲缘关系特征。从64对引物组合中筛选出9对重复性和稳定性好的引物组合,总共扩增出399条清晰条带,即对枣品种基因组399个位点进行了检测,其中共有位点85个,多态性位点314个,多态性百分率78.70%,可鉴别出所有供试品种。UPGMA聚类表明,30个枣树品种的相似系数在0.4654~ 0.8231之间,平均相似系数为0.6223,在相似系数0.666处,可将供试的30个枣树品种分为6类,为枣树品种分类及亲缘关系分析提供了有力参考。

关键词: 农业科技期刊, 农业科技期刊, 存在问题, 复合型, 青年编辑, 培养, 必要性, 途径

Abstract: In order to acquire the relationship of Chinese jujube cultivars, 30 main Chinese jujube cultivars were analyzed by using AFLP technique and the genetic clustering analysis were also be studied. 9 primer pairs with best repeatability and stability were selected from 64 primer pairs, 399 bands were generated in total, in which 85 bands were mutual and 314 bands were polymorphic; the average percentage of polymorphic band was 78.70%. Then all of the 30 Chinese jujubes could be identified. The UPMGA showed the similarity coefficients were between 0.4654 and 0.8231, and the average similarity coefficient was 0.6223, The 30 cultivars and strains can be divided into 6 groups according to cluster analysis while the similarity was 0.666. These results provided a strong reference on the Chinese jujube cultivar classification and relationship analysis.