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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (13): 45-50.doi: 10.11924/j.issn.1000-6850.2013-2948

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

黄花矶松组织培养及培养基筛选研究

王方琳 崔建国 尉秋实 张莹花 柴成武   

  • 收稿日期:2013-11-10 修回日期:2014-01-10 出版日期:2014-05-05 发布日期:2014-05-05
  • 基金资助:
    国家科技计划(2012“)祁连山水源涵养林生态系统保育技术试验示范”(BAD16B00)；国家自然科学基金“沙区粘土质夹层土壤水分分布与植物的生态响应”(31100519)。

Research on Tissue Culture and Medium of Limoniumau-reum

  • Received:2013-11-10 Revised:2014-01-10 Online:2014-05-05 Published:2014-05-05

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摘要： 试验选用种子培养的黄花矶松无菌苗的子叶、茎段、下胚轴作为外植体材料，研究不同外植体的离体培养技术及其适宜的培养基。结果表明，生长素2,4-D对不定芽诱导具有明显的促进作用，在其浓度为1.5 mg/L时诱导率最高，子叶是诱导不定芽的良好外植体，最适培养基为MS+ 2,4-D 1.5 mg/L+ 6-BA 2.0 mg/L+ NAA 1.0 mg/L。黄花矶松的最适增殖培养基为MS+6-BA 1.0 mg/L+ NAA 1.0 mg/L，而且是以丛生芽的方式进行增殖的；最适生根培养基是1/2 MS+ KT 1.0 mg/L+ IBA 1.0 mg/L。

关键词: 预警, 预警

Abstract: Experiment chose seed development Limoniumau- reum no vaccine hypocotyl cotyledon stem section as explant materials, study the culture of different explant technique, the results showed that: the suitable medium auxin 2,4- D on adventitious bud induction had an obvious role in promoting, when its concentration was 1.5 mg/L induction rate was highest, cotyledon was good explant of adventitious bud induction, the optimal culture medium for MS+2,4-D 1.5 mg/L+6-BA 2.0 mg/L+NAA 1.0 mg/L. Limoniumaureum optimal proliferation medium was MS+6-BA 1.0 mg/L+ NAA 1.0 mg/L, and took the form of multiple shoot clumps proliferation, The optimum rooting medium was 1/2 MS+ KT 1.0 mg/L+ IBA 1.0 mg/L.