IBA对兔眼蓝浆果嫩枝扦插生根及内源激素变化的影响

doi:10.11924/j.issn.1000-6850.2013-2984

中国农学通报 ›› 2014, Vol. 30 ›› Issue (16): 123-130.doi: 10.11924/j.issn.1000-6850.2013-2984

所属专题：生物技术

IBA对兔眼蓝浆果嫩枝扦插生根及内源激素变化的影响

宋鹏飞陈华江姜燕琴韦继光李建华曹雪丹

收稿日期:2013-11-13 修回日期:2014-01-22 出版日期:2014-06-05 发布日期:2014-06-05
基金资助:
国家农业部公益性行业（农业）科研专项“小浆果产业技术研究与试验示范”(201103037)；江苏省农业科技自主创新项目“南方高丛蓝浆果早熟新优品系的选育”[CX(11)1011]。

Effect of different concentrations of IBA on Changes of Endogenous Hormones Levels and Adventitious Root Formation in Softwood Cutting of Rabbiteye Blueberry

Received:2013-11-13 Revised:2014-01-22 Online:2014-06-05 Published:2014-06-05

摘要/Abstract

摘要： 以兔眼蓝浆果品种‘粉蓝’为试材，研究不同质量浓度(0、3000、5000、7000、9000 mg/L)吲哚丁酸(IBA)处理对‘粉蓝’嫩枝扦插生根及内源激素变化动态的影响。结果表明：兔眼蓝浆果品种‘粉蓝’嫩枝插条生根能力随外源IBA浓度增加而增强；扦插过程中适宜浓度内源IAA及IAA/GA有利于插条愈伤组织和根原基分化形成，高浓度内源GA抑制‘粉蓝’插条不定根的形成；在愈伤组织及根原基形成期ZT含量降低、IAA/ZT升高促进根原基分化形成，而在生根中后期高浓度ZT、低IAA/ZT有利于细胞分裂和不定根伸长生长。ABA及IAA/ABA在生根过程中没有明显变化。

关键词: 冻害指数, 冻害指数

Abstract: The dynamics of endogenous hormones in softwood cuttings of rabbiteye blueberry (Vaccinium ashei Reade) cultivar‘Powderblue’, treated by five levels of indolebutyric acid(IBA) (0, 3000, 5000, 7000 and 9000 mg/L) were studied during adventitious root formation. The results showed that exogenous IBA increased the rooting ability of softwood cuttings of rabbiteye blueberry. Suitable levels of endogenous IAA and IAA/GA were beneficial to the formation of callus tissue and root primordium, and endogenous GA at higher level inhibited formation of adventitious root in cuttings. The function of endogenous ZT and IAA/ZT were different during different cutting periods. Low level of endogenous ZT and high level of IAA/ZT promoted differentiation and formation of root primordium during the formation of callus tissue and root primordium stage. High level of endogenous ZT and low level of IAA/ZT favored the elongation and growth of adventitious roots in cuttings. The changes of ABA and IAA/ABA were not obvious.

宋鹏飞陈华江姜燕琴韦继光李建华曹雪丹. IBA对兔眼蓝浆果嫩枝扦插生根及内源激素变化的影响[J]. 中国农学通报, 2014, 30(16): 123-130.

参考文献

[1] Sun L Q, Ding X P, Qi J, et al Antioxidant anthocyanins screening through spectrum – effect relationships and DPPH- HPLC- DAD analysis on nine cultivars of introduced rabbiteye blueberry in China [J]. Food Chemistry,2012,132:759-765.
[2] 张慧琴,谢鸣,梁英龙,等.我国蓝莓研发现状及产业化发展潜在优势[J].浙江农业科学,2009,3:444-447.
[3] 於虹,顾姻,贺善安.我国南方地区越橘栽培现状及发展展望[J].中国果树,2009,3:68-70,72.
[4] 史海芝,刘惠民.兔眼蓝莓嫩枝扦插繁殖技术[J].西南林学院学报, 2010,30(2):25-27+32.
[5] Spiers J M, Braswell J H, Hegwood C P. Establishment and maintenance of rabbiteye blueberries[M]. Mississippi Agricultural & Forestry Experiment Station,1985:9-10.
[6] 韦吉梅,聂飞,何健,等.不同处理措施对兔眼蓝莓绿枝扦插生根的影响[J].落叶果树,2008,5:12-14.
[7] Vandergriff D, Witte W T. Penetrating agents for liquid quick-dip rooting hormones[J]. HortScience,1995,30(4):805.
[8] 齐永顺,张志华,王同坤,等.同源四倍体玫瑰香葡萄嫩枝扦插不定根发生过程中内源激素的变化[J].园艺学报,2009,36(4):565-570.
[9] 于玉梅,刘春香,朱妍妍,等.高效液相色谱法在黄瓜果实内源激素测定上的应用及改进[J].山东农业科学,2008,(7):97-99.
[10] 李永欣,曾慧杰,王晓明,等.光皮树扦插过程中内源激素变化[J].中国农学通报,2010,26(15):247-251.
[11] 袁利利,张林,王厚新,等.华北五角枫‘京2’插穗生根过程中内源激素变化[J].中国农学通报,2012,28(13):61-64.
[12] Blakesley D, Chaldecott M A. The role of endogenous auxin in root initiation. II: Sensitivity, and evidence from studies on transgenic plant tissues[J]. Plant growth regulation,1993,13(1):77-84.
[13] 赵云龙,陈训,李朝婵.糙叶杜鹃扦插生根过程中生理生化分析[J].林业科学,2013,49(6):45-51.
[14] 张芹,李保会,朵建国,等.IBA和发根农杆菌对糠椴嫩枝扦插的影响[J].园艺学报,2007,34(1):201-204.
[15] 郭英超,杜克久,贾哲.兴安圆柏扦插生根过程中相关内源激素特征分析[J].中国农学通报,2012,28(1):44-48.
[16] Brian P W, Hemming H G, Lowe D. Inhibition of rooting of cuttings by gibberellic acid: with one figure in the text[J]. Annals of Botany,1960,24(4):407-419.
[17] Bollmark M, Eliasson L. Effects of exogenous cytokinins on root formation in pea cuttings[J]. Physiologia Plantarum,1986,68,662- 666.
[18] 王乔春.植物激素与插条不定根的形成[J].四川农业大学学报, 1992,10(1):33-39.
[19] Bollmark M, Kubát B, Eliasson L. Variation in Endogenous Cytokinin Content during Adventitious Root Formation in Pea Cuttings[J]. Journal of Plant Physiology,1988,132:262-265.
[20] 郭素娟,凌宏勤,李凤兰.白皮松插穗生根的生理生化基础研究[J].北京林业大学学报,2004,26(2):43-48.
[21] 师晨娟,刘勇,王春城,等.青海云杉扦插的年龄效应及其生根机理研究[J].西北农林科技大学学报:自然科学版,2006,34(12):101- 104.
[22] 杜蕊.矮生彩色针叶树扦插繁殖年龄效应及栽培技术研究[D].北京:北京林业大学,2012:37.

IBA对兔眼蓝浆果嫩枝扦插生根及内源激素变化的影响

Effect of different concentrations of IBA on Changes of Endogenous Hormones Levels and Adventitious Root Formation in Softwood Cutting of Rabbiteye Blueberry

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	钟意,庄舜尧,何钧潮,桂仁意. 集约经营措施对雷竹冻害程度的影响[J]. 中国农学通报, 2018, 34(5): 95-100.
[2]	张媛,李宗波. 2013年昆明市榕属植物冻害及恢复研究[J]. 中国农学通报, 2016, 32(4): 21-25.
[3]	马晓辉刘燕王良民. 19种园林绿化树木1年生播种苗越冬研究[J]. 中国农学通报, 2012, 28(34): 102-107.