欢迎访问《中国农学通报》,

中国农学通报 ›› 2014, Vol. 30 ›› Issue (16): 252-255.doi: 10.11924/j.issn.1000-6850.2013-3137

• 林学 园艺 园林 • 上一篇    下一篇

利用生物反应器大量生产东北刺人参不定根的研究

于丹 廉美兰   

  • 收稿日期:2013-12-02 修回日期:2014-01-17 出版日期:2014-06-05 发布日期:2014-06-05
  • 基金资助:
    国家自然科学基金 “东北刺人参不定根反应器培养机制及抗氧化特性研究” (31260182)。

Mass Production of Adventitious Roots of O. elatus in Bioreactor

  • Received:2013-12-02 Revised:2014-01-17 Online:2014-06-05 Published:2014-06-05

摘要: 为了大量生产东北刺人参不定根,研究了培养方式和反应器内培养基中IBA浓度、糖种类以及白糖浓度对其不定根增殖生长的影响,结果表明生物反应器可大量生产东北刺人参不定根;在反应器培养时,MS培养基中添加3 mg/L的IBA时不定根长势较好,其鲜物重、干物重最大,分别达到378.3 g、34.8 g;而白糖和蔗糖对不定根的增殖效果显著好于葡萄糖和果糖,因此,选用低价的白糖作为碳源,白糖浓度为50 g/L时其鲜物重、干物重以及增殖系数达到最大,分别为551.9 g、57.2 g和26.6。

关键词: 示范区规划, 示范区规划, 规划指标体系

Abstract: For mass production of adventitious roots of Oplopanax elatus Nakai, the effects of culture methods, IBA concentrations, sugar types and white sugar concentrations during bioreactor on adventitious roots proliferation and growth were studied. The results showed that bioreactor culture was optimal. The best adventitious roots proliferation was observed when 3 mg/L IBA was added into the medium during bioreactor culture, the fresh and dry weight reached the maximum (378.3g, 34.8g). White sugar and sucrose were better than glucose and fructose in adventitious roots proliferation, therefore the cheap white sugar was regarded as carbon source, and white sugar concentration of 50 g/L was the most suitable for fresh and dry weight as well as multiplication coefficient, the maximum were 551.9 g、57.2 g and 26.6, respectively.