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中国农学通报 ›› 2015, Vol. 31 ›› Issue (21): 144-148.doi: 10.11924/j.issn.1000-6850.2014-2274

所属专题: 生物技术

• 生物技术科学 • 上一篇    下一篇

油棕抗寒性与内源激素的关系

刘世红   

  1. 云南省热带作物科学研究所
  • 收稿日期:2014-08-20 修回日期:2015-06-29 接受日期:2015-05-25 出版日期:2015-07-28 发布日期:2015-07-28
  • 通讯作者: 刘世红
  • 基金资助:
    农业部南亚热作专项 “油棕区域适应性试种” (2013RZNJ); 科研基本业务费项目 “云南省热带作物科技创新体系建设资金” (RF2014-11)。

Relationship Between Cold-resistance and Endogenous Hormone in Oil Palm

  • Received:2014-08-20 Revised:2015-06-29 Accepted:2015-05-25 Online:2015-07-28 Published:2015-07-28

摘要: 为研究油棕抗寒性与内源激素的关系,人工设置温度梯度,模拟Logistic方程,求取低温半致死温度,鉴定抗寒性,采用ELISA技术测定自然降温过程中内源激素的含量。结果表明,4个品种低温半致死温度分别为-4.1℃(T1)、-0.92℃(T2)、-2.89℃(T3)、2.18℃(M1),抗寒能力为T1>T3>T2>M1。自然降温过程中,从26℃降到18℃,IAA、GA、CTK、ABA含量基本不变,低于18℃后,IAA、ABA含量显著增加,GA含量稍有增加,CTK含量降低,ETH含量则变化不规律;ABA/CTK比值升高,ABA/IAA、GA/IAA、CTK/IAA、ETH/IAA比值先升高后降低。抗寒性高的品种,IAA、ABA含量高,IAA/ABA、IAA/ETH、GA/ETH、CTK/ETH、ABA/ETH比值高,表明油棕叶片IAA和ABA含量的增加利于提高抗寒力。

关键词: 干重, 干重

Abstract: For studying the relationship between cold- resistance and endogenous hormones, temperature gradient was set artificially and Logistic equation was simulated to calculate the semilethal temperature of low temperature and identify cold-resistance, and EILAS technique was adopted to test the content of endogenous hormones in the process of natural cooling. The results showed that semilethal temperature of four tested varieties was -4.1℃(T1), -0.92℃(T2), -2.89℃(T3), 2.18℃(M1), respectively, the order of cold tolerance was T1> T3>T2>M1. In the whole process of natural temperature decrease, the content of IAA, GA, CTK and ABA of varieties T1, T2, T3 and M1 basically unchanged when the temperature decreased from 26℃ to 18℃, while when the temperature was below 18℃, IAA and ABA content increased, GA content increased slightly, CTK content decreased, and the change of ETH was irregular; ABA/CTK ratio increased, and the ratio of ABA, GA, CTK and ETH to IAA first increased and then decreased. Varieties with high cold resistance had high content of IAA and ABA, and high ratio of IAA/ABA, IAA/ETH, GA/ETH, CTK/ETH and ABA/ETH, which showed that the increase of the content of IAA and ABA of oil palm leaves could improve cold resistance.