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中国农学通报

中国农学通报 ›› 2017, Vol. 33 ›› Issue (33): 135-141.doi: 10.11924/j.issn.1000-6850.casb17020049

• 植物保护 农药 • 上一篇    下一篇

藤仓赤霉菌的抗药性及对不同杀菌剂敏感性的相关分析

陈宏州,杨红福,姚克兵,庄义庆,缪康,束兆林   

  1. 江苏丘陵地区镇江农业科学研究所,江苏丘陵地区镇江农业科学研究所,江苏丘陵地区镇江农业科学研究所,江苏丘陵地区镇江农业科学研究所,江苏丘陵地区镇江农业科学研究所,江苏丘陵地区镇江农业科学研究所
  • 收稿日期:2017-02-15 修回日期:2017-03-13 接受日期:2017-03-22 出版日期:2017-11-27 发布日期:2017-11-27
  • 通讯作者: 束兆林
  • 基金资助:
    江苏省第四期333 工程科研资助项目“水稻种传病害抗药性监测及安全防控技术研究”(BRA2015206)；镇江市农业科技支撑项目“水稻种 传病害抗药性监测及安全防控技术研究”(NY2015021)

Drug Resistance of Gibberella fujikuroi and Correlation Analysis of Its Sensitivity to Different Fungicides

  • Received:2017-02-15 Revised:2017-03-13 Accepted:2017-03-22 Online:2017-11-27 Published:2017-11-27

摘要： 为探明江苏省藤仓赤霉菌(Gibberella fujikuroi)对多菌灵、咪鲜胺和氰烯菌酯的抗药性以及多菌灵与咪鲜胺对其它杀菌剂的交互抗性,采用区分剂量法分别检测了采自江苏省16个县(市)的202株藤仓赤霉菌对多菌灵、咪鲜胺和氰烯菌酯的抗药性,并采用菌丝生长速率法分别检测了对多菌灵与咪鲜胺不同敏感性的10个菌株对戊唑醇、灭菌唑、丙硫菌唑、叶菌唑、咯菌腈和氟啶胺等杀菌剂的敏感性。结果表明,江苏省藤仓赤霉菌对多菌灵、咪鲜胺和氰烯菌酯的抗性频率分别为77.72%、67.33%和0%。根据菌株对3种杀菌剂的抗药性表现,可分为4种表现型,其中对多菌灵和咪鲜胺抗药而对氰烯菌酯敏感的菌株占58.42%；对多菌灵抗药而对咪鲜胺和氰烯菌酯敏感的菌株占20.30%；对咪鲜胺抗药而对多菌灵和氰烯菌酯敏感的菌株占9.90%；对多菌灵、咪鲜胺和氰烯菌酯均敏感的菌株占11.39%。通过敏感性相关性分析,藤仓赤霉菌对多菌灵以及咪鲜胺和上述杀菌剂之间均无交互抗性。江苏省藤仓赤霉菌对多菌灵和咪鲜胺的抗性频率较高而对氰烯菌酯敏感,需要筛选新的杀菌剂防治水稻恶苗病。

关键词: 木薯渣, 木薯渣, 堆肥, 氮素转化, 腐熟度

Abstract: To investigate the resistance of Gibberella fujikuroi collected from Jiangsu province to carbendazim, prochloraz and phenamacril as well as the cross-resistance of carbendazim and prochloraz to other fungicides, the resistance of 202 isolates of G.fujikuroi collected from 16 counties(cities) in Jiangsu province to carbendazim, prochloraz and phenamacril have been detected by using distinguishing dosage method, respectively, and then 10 isolates with different sensitivity to carbendazim and prochloraz were used for cross-resistance detection to tebuconazole,triticonazole, prothioconazole, metconazole,fludioxonil and fluazinam by using mycelium growth rate method,respectively. The results of resistance detection showed that the resistance frequency of G.fujikuroi collected from Jiangsu province to carbendazim, prochloraz and phenamacril were 77.72%, 67.33% and 0%, respectively. According to the resistance phenotypes of all isolates to 3 fungicides, 4 resistance types of G.fujikuroi have been identified.The isolates resistant to carbendazim and prochloraz and sensitive to phenamacril accounted for 58.42%, the isolates resistant to carbendazim and sensitive to prochloraz and phenamacril accounted for 20.30%, the isolates resistant to prochloraz and sensitive to carbendazim and phenamacril accounted for 9.90%, the isolates sensitive to carbendazim, prochloraz and phenamacril accounted for 11.39%. The results of sensitivity correlateon analysis showed that there were no positive-correlation across resistance between carbendazim and other fungicides as well as prochloraz and other fungicides. These results show that G.fujikuroi in Jiangsu province have relatively high resistance frequency to carbendazim and prochloraz, but were sensitive to phenamacril, and it’s need to select new fungicide to control rice bakanae disease.