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中国农学通报

中国农学通报 ›› 2021, Vol. 37 ›› Issue (6): 111-116.doi: 10.11924/j.issn.1000-6850.casb2020-0156

所属专题： 生物技术

• 生物科学 • 上一篇    下一篇

遮阴和露地栽培条件下蜘蛛香叶片的抗氧化酶比活力和丙二醛含量

李桂琼1(), 赵鹏宇2, 赵昶灵1(), 张洪玲1(), 臧灵飞1, 文国松1, 徐绍忠1, 张金渝3, 杨斌3, 杨绍兵3, 庄天才4   

  1. 1云南农业大学农学与生物技术学院,昆明 650201
    2上海理工大学环境与建筑学院,上海 200093
    3云南省农业科学院药用植物研究所,昆明 650200
    4云南生绿云生物科技有限公司,昆明 650118
  • 收稿日期:2020-06-08 修回日期:2020-08-10 出版日期:2021-02-25 发布日期:2021-02-24
  • 通讯作者: 赵昶灵,张洪玲
  • 作者简介:李桂琼,女,1989年出生,云南弥勒人,博士生,主要从事药用植物生态生理研究。通信地址：650201 云南省昆明市盘龙区沣源路452号 云南农业大学农学与生物技术学院,Tel：0871-65227213,E-mail：762039906@qq.com。
  • 基金资助:
    云南省生物医药重大科技专项“滇红花、川贝母等五种中药材种子种苗繁育基地建设关键技术研究及应用”子课题七“蜘蛛香种子种苗繁育基地建设关键技术研究及应用”(2018ZF010-1)

Specific Activities of Antioxidases and Malondialdehyde Contents of Valeriana jatamansi Jones Leaves Under Shading and Open Field Cultivation

Li Guiqiong1(), Zhao Pengyu2, Zhao Changling1(), Zhang Hongling1(), Zang Lingfei1, Wen Guosong1, Xu Shaozhong1, Zhang Jinyu3, Yang Bin3, Yang Shaobing3, Zhuang Tiancai4   

  1. 1College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201
    2College of Environment and Architecture, University of Shanghai for Science and Technology, Shanghai 200093
    3Institute of Medicinal Plant, Yunnan Academy of Agricultural Sciences, Kunming 650200
    4Yunnan ShengLüYun Biotechnology Corporation Ltd., Kunming 650118
  • Received:2020-06-08 Revised:2020-08-10 Online:2021-02-25 Published:2021-02-24
  • Contact: Zhao Changling,Zhang Hongling

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摘要：

研究旨在阐明蜘蛛香叶片酶类抗氧化能力在遮阴和露地栽培条件下的差异,为蜘蛛香应答阳生环境的研究和蜘蛛香大田栽培配套技术的制定提供参考。以滇中地区遮阴和露地栽培条件下的蜘蛛香叶片为试材,用分光光度法研究了叶片的可溶性蛋白质含量和抗氧化酶[（即：过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)]的比活力以及丙二醛(MDA)含量。与遮阴栽培相比,露地栽培会使蜘蛛香叶片的可溶性蛋白质含量降低了11.30%,但会使叶片CAT、POD和SOD比活力以及MDA含量分别升高了11.43%、30.90%、21.58%和18.48%;叶片可溶性蛋白质含量和CAT比活力在遮阴和露地条件下的差异均未达到显著水平,而POD和SOD比活力以及MDA含量的差异均达到显著水平。在露地栽培条件下,蜘蛛香叶片的可溶性蛋白质含量下降、细胞脂质的过氧化损伤加剧,但CAT、POD和SOD比活力会同时提高而强化叶片细胞的酶类抗氧化能力。

关键词: 蜘蛛香叶片, 抗氧化酶比活力, 可溶性蛋白质含量, 丙二醛含量, 遮阴和露地栽培

Abstract:

This research aims to elucidate the differences of the enzymatic antioxidant abilities of Valeriana jatamansi Jones leaves under the conditions of shading and open field cultivation, and to provide a reference for studying the responses of V. jatamansi to sunny environments and formulating the supporting techniques of field cultivation of V. jatamansi. The V. jatamansi leaves under the conditions of the shading and open field in the central part of Yunnan Province were used as the test materials, and the soluble protein content and the specific activities of the antioxidases, i.e., catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD), and the malondialdehyde (MDA) content of the leaves were determined with spectrophotometry. Compared with the shading condition, the open field condition decreased the soluble protein content of the V. jatamansi leaves by 11.30%, but increased the specific activities of the CAT, POD and SOD, and the MDA content of the leaves by 11.43%, 30.90%, 21.58% and 18.48%, respectively. The differences of the soluble protein content and CAT specific activities of the leaves under the condition of the shading and open field cultivation did not reach the significant level, whereas those of the specific activities of the POD and SOD and the MDA content all reached the significant level. Under the conditions of open field cultivation, the soluble protein contents of the V. jatamansi leaves declined and the peroxidation-induced damages of the cellular lipids became worse, but the specific activities of the CAT, POD and SOD rose simultaneously to strengthen the enzymatic antioxidant ability of the leaf cells.

Key words: leaves of Valeriana jatamansi Jones, specific activities of antioxidases, soluble protein content, malondialdehyde content, shading and open field cultivation

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