低温胁迫对甜菜抗氧化系统的影响

doi:10.11924/j.issn.1000-6850.casb2021-0844

中国农学通报 ›› 2022, Vol. 38 ›› Issue (12): 26-32.doi: 10.11924/j.issn.1000-6850.casb2021-0844

所属专题：生物技术；园艺

低温胁迫对甜菜抗氧化系统的影响

孙佳平¹^,²(), 张福顺³^,⁴(), 邳植³^,⁴, 周芹³^,⁴

¹黑龙江大学中俄联合研究生院,哈尔滨 150080
²黑龙江大学生命科学学院黑龙江省寒地生态修复与资源利用重点实验室,哈尔滨 150080
³黑龙江大学现代农业与生态环境学院,哈尔滨 150080
⁴农业农村部甜菜品质监督检验测试中心,哈尔滨 150080

收稿日期:2021-07-13 修回日期:2021-10-13 出版日期:2022-04-25 发布日期:2022-05-18
通讯作者: 张福顺
作者简介:孙佳平,女,1997年出生,黑龙江绥化人,硕士研究生,研究方向：植物生态学。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学农学楼,Tel：13904839158,E-mail： 2809506362@qq.com。
基金资助:
农作物种质资源保护与利用专项“甜菜种质资源的收集、鉴定、编目、繁种与入库（圃）保存(1921-26);现代农业产业技术体系建设专项资金资助(CARS-170503)

Effect of Low Temperature Stress on the Antioxidant System of Sugar Beet

SUN Jiaping¹^,²(), ZHANG Fushun³^,⁴(), PI Zhi³^,⁴, Zhou Qin³^,⁴

¹Sino-Russian Joint Graduate School of Heilongjiang University, Harbin 150080
²Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization in Cold Regions, College of Life Sciences, Heilongjiang University, Harbin 150080
³College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080
⁴Sugarbeet Quality Supervision, Inspection and Testing Center of Ministry of Agriculture and Rural Affairs, Harbin 150080

Received:2021-07-13 Revised:2021-10-13 Online:2022-04-25 Published:2022-05-18
Contact: ZHANG Fushun

摘要/Abstract

摘要：

旨在研究低温胁迫对甜菜细胞膜过氧化及抗氧化系统的影响,并为研究其他植物的抗寒性生理机制提供参考依据。以甜菜品种‘KWS9147'为实验材料,采用水培方式栽培,设置了4℃的低温处理(L)和25℃的室温对照(CK),测定低温处理前后甜菜叶片中丙二醛(MDA)含量和抗氧化系统酶活性的变化。结果表明,低温胁迫会对细胞膜造成损害并促使抗氧化系统产生防御作用,甜菜低温胁迫处理1天后与对照组对比,丙二醛(MDA)含量和抗氧化系统酶活性发生了显著的变化,丙二醛(MDA)的含量增加了31.32%,总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)及过氧化氢酶(CAT)活性显著上升,分别上升了10.74%、4倍、2.22倍、32.85%、和2.56倍。综上,甜菜在低温胁迫下抗氧化能力提高,从而清除活性氧自由基(ROS)的积累,减缓细胞膜脂过氧化对细胞造成的伤害。

关键词: 低温, 甜菜, 丙二醛, 抗氧化酶, 细胞膜损伤

Abstract:

The aims of this study are to investigate the effects of low temperature stress on cell membrane peroxidation and antioxidant system in sugar beet, and provide reference for studying physiological mechanism of cold resistance in other plants. Sugar beet variety ‘KWS9147' was selected as the experimental material and cultivated in hydroponic culture. The low temperature (4℃) was used as stress treatment (L) and the room temperature (25℃) was used as control (CK). The changes of malondialdehyde (MDA) content and antioxidant system enzyme activity in sugar beet leaves before and after low temperature treatment were measured. The results showed that low temperature stress damaged the cell membrane and promoted the defense role of the antioxidant system. Compared with CK, the content of malondialdehyde (MDA) and the enzyme activity of the antioxidant system changed significantly 1 day after low temperature stress. The content of MDA increased by 31.32%, and the activities of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), peroxidase (POD), ascorbic acid peroxidase (APX) and catalase (CAT) increased significantly by 10.74%, 4 times, 2.22 times, 32.85% and 2.56 times, respectively. In summary, the antioxidant capacity of sugar beet was improved for scavenging the accumulation of reactive oxygen free radicals (ROS) and alleviating membrane damage caused by membrane lipid peroxidation under low temperature stress.

Key words: low temperature, sugar beet, malondialdehyde, antioxidant enzymes, cell membrane damage

中图分类号:

S566.3

孙佳平, 张福顺, 邳植, 周芹. 低温胁迫对甜菜抗氧化系统的影响[J]. 中国农学通报, 2022, 38(12): 26-32.

SUN Jiaping, ZHANG Fushun, PI Zhi, Zhou Qin. Effect of Low Temperature Stress on the Antioxidant System of Sugar Beet[J]. Chinese Agricultural Science Bulletin, 2022, 38(12): 26-32.

图/表 4

图1. 低温胁迫对甜菜形态特征的影响

图2. 低温胁迫对甜菜叶片MDA含量的影响不同小写字母表示差异显著(P<0.05)

图3. 低温胁迫对甜菜叶片T-AOC的影响不同小写字母表示差异显著(P <0.05)

图4. 低温胁迫对甜菜叶片4种抗氧化酶活性的影响 A：SOD活性,B：POD活性,C：APX活性,D：CAT活性;不同小写字母表示差异显著(P <0.05)

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低温胁迫对甜菜抗氧化系统的影响

Effect of Low Temperature Stress on the Antioxidant System of Sugar Beet

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