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农学学报

农学学报 ›› 2013, Vol. 3 ›› Issue (3): 54-56.

所属专题： 生物技术

• 林学 园艺 园林 食用菌 • 上一篇    下一篇

大叶藻组织培养中外植体消毒方法的研究

刘延岭 李晓捷 崔翠菊 王娜 赛珊 李琪   

  • 收稿日期:2013-01-11 修回日期:2013-02-02 出版日期:2013-03-20 发布日期:2013-03-20
  • 基金资助:
    烟台市科技发展计划项目

Study on the disinfection method for tissue culture of Zostera marina

  • Received:2013-01-11 Revised:2013-02-02 Online:2013-03-20 Published:2013-03-20

摘要： 为探寻大叶藻组织培养中外植体的最佳消毒方法，采用75%乙醇、1.5%碘化钾、4%次氯酸钠和10%双氧水这4种消毒剂以及不同的处理时间组合，对大叶藻种子和幼苗进行消毒试验。试验结果，最有效的外植体消毒方法为：对大叶藻幼苗，先用无菌水冲洗3~4次，再以75%乙醇处理20 s，然后再用无菌水冲洗3~4次，可以达到较好的消毒效果。对大叶藻种子，先以75%乙醇处理10 min，再以1.5%碘化钾处理10 min，然后用无菌海水冲洗3~4次，这样消毒效果最佳，且不影响种子的发芽率。

关键词: 高粱, 高粱, 胚轴, 胚芽鞘, 聚类分析

Abstract: In order to establish the suitable disinfection method for tissue culture of Zoster marina. 75% ethanol, 1.5% potassium iodide, 4% sodium hypochlorite and 10% hydrogen peroxide as well as different disinfection time were used for seeds and seedlings sterilization treatment. The results indicated that for seedlings, sterile sea water rinsed for 3-4 times, 75% ethanol sterilized for 20 s, then sterile sea water rinsed for 3-4 times, which achieved good sterilizing effects. The efficient disinfection method for seeds was that, 75% ethanol rinsed for 10 min, 1.5% potassium iodide immersed for 10 min and then sterile sea water rinsed for 3-4 times, which showed the optimal disinfection effects and did not affect seed germination rate.