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农学学报

农学学报 ›› 2014, Vol. 4 ›› Issue (5): 67-69.

所属专题： 生物技术

• 农业工程 农业机械 生物技术 食品科学 • 上一篇    下一篇

不同激素浓度对食用菊花组织培养的影响

刘玉堂 赵宪争 杨迎霞 岳东霞 尉万聪   

  • 收稿日期:2013-11-18 修回日期:2013-12-18 出版日期:2014-05-20 发布日期:2014-05-20
  • 基金资助:
    药用植物资源引进及优良新品系的选育

Effects of Different Plant Hormone Concentrations to Tissue Culture Stem of Edible Chrysanthemum

  • Received:2013-11-18 Revised:2013-12-18 Online:2014-05-20 Published:2014-05-20

PDF (PC)

1177

摘要： 以盆栽食用菊花的茎尖为外植体，通过调节激素浓度诱导愈伤形成、芽体萌发、继代增殖以及生根壮苗，快速扩繁食用菊花组培苗。研究结果表明：愈伤分化和芽诱导的最适培养基为MS+ 1 mg/L 6-BA+ 0.1 mg/L NAA，芽的诱导率可达到55%；不定芽增殖的最适培养基为MS+ 1 mg/L 6-BA+ 0.3 mg/L NAA+ 0.1 mg/L KT，平均每株芽数4.9个；生根的最适培养基为1/2MS+ 0.1 mg/L NAA，根数最多可达11根。

关键词: 植株, 植株, 叶绿素 无损诊断技术

Abstract: With potted edible chrysanthemum shoot tip explants, by adjusting the concentration of hormone induced callus formation、bud germination、subculture and the rooting, then the edible chrysanthemum are propagated rapidly. The results showed that MS+ 1 mg/L 6-BA+ 0.1 mg/L NAA was the best medium for callus and sprout differentiation. The induction of sprout was 55%. In the process of proliferation for plant tall and sturdy stems and leaves, MS+ 1 mg/L 6-BA+ 0.3 mg/L NAA+ 0.1 mg/L KT was the medium better. The optimum rooting medium was 1/2MS+ 0.1 mg/L NAA. Root number up to 11.