不同草莓品种酚类物质含量及抗氧化能力研究

doi:10.11923/j.issn.2095-4050.cjas17070013

摘要/Abstract

摘要： 【目的】为研究山西晋中地区草莓不同品种酚类物质含量及其抗氧化能力,并为草莓栽培、育种、加工利用提供相关依据。【方法】采用 Folin-Ciocalteu 法测定总酚含量,Folin-Denis法测定单宁酚含量；正丁醇-盐酸法测定原花色素含量；香草醛-硫酸比色法测定花青素含量；采用羟基自由基清除力、DPPH自由基清除力和FRAP铁还原值三种方法测定抗氧化能力。【结果】结果表明：枥乙女的总酚、单宁酚、花青素、原花色素含量均为最高；综合3个抗氧化性指标,枥乙女的抗氧化能力较强；主成分分析表明,前三个主成分基本概括了酚类物质含量和抗氧化能力的主要信息,其累积贡献率为92.59%。【结论】16个草莓品种中,枥乙女综合评分最高,是促进草莓消费市场和产业发展重要品种资源。

关键词: 断根, 断根, 控稍, 叶部病害, 早期落叶

Abstract: To study the content of phenolic compounds and antioxidant capacity of different strawberry varieties in Jinzhong Prefecture of Shanxi Province while provide a basis for the reasonable development and effective utilization of plant polyphenols in strawberry, total phenols content was determined by Folin- Ciocalteu method, tannin phenol content was determined by Folin-Denis method, proanthocyanidin content was determined by inn- butanol- HCl method, anthocyania content was determined by vanillin- sulfuric colorimetry, antioxidant activity was determined by hydroxyl radical scavenging method, DPPH radical scavenging method and FRAP iron reducing method, and then the data were mined by variation analysis, correlation analysis and principal component analysis. It was indicated that the content of total phenol, tannin, anthocyanin, and proanthocyanidin in‘Tochiotome’was the highest while that of‘05-1-5’was the lowest, antioxidant capacity of‘Tochiotome’was the highest while that of‘05- 1- 5’was the lowest through comprehensive analysis of 3 antioxidant indexes, phenolics content and antioxidant capacity of strawberry were significantly correlated, and by principal component analysis it was showed that the first three principal components basically outlined the main information of phenolics content and antioxidant capacity with a cumulative contribution rate of 92.59%, and‘Tochiotome’had the best comprehensive quality. In conclusion, ‘Tochiotome’is an important genetic resource to cultivate new strawberry varieties with high biological activity and to promote the development of strawberry industry.

中图分类号:

S661.1

曹贵寿,张倩茹,尹蓉,茹慧玲,李小平,王贤萍,王俊宇. 不同草莓品种酚类物质含量及抗氧化能力研究[J]. 农学学报, 2018, 8(2): 35-41.

参考文献

[1] 花秀凤.草莓种质资源鉴定和种质创新研究[D].福州:福建农林大学,2012.HUA Xiufeng. Strawberry Germplasm resource identificatian and studies on Germplasm Innovation[D].Fuzhou: Fujian Agriculture and Forestry University, 2012.
[2] 王俊宇,梁艳霞,刘刚,张拥兵,曹贵寿. 山西省草莓种质资源研究与利用[J].黑龙江农业科学,2016, (3):151-153.WANG Junyu, LIANG Yanxia, LIU Gang.ZHANG Yongbing,CAO Guishou.Research and Utilization of Strawberry Germplasms in Shanxi Province[J]. Heilongjiang Agricultural Sciences, 2016, (3):151-153.
[3] MEYERS K J, WATKINS C B, PRITTS M P, LIU R H. Antioxidant and antiproliferative activities of strawberries[J].Journal of Agricultural and Food Chemistry,2003,51(23) : 6887-6892.
[4] WANG H, CAO G H, RONALD L. PRIOR. Total antioxidant capacity of fruits[J].Journal of Agricultural and Food Chemistry,1996,44(3) :701-705.
[5] HENNING S M, SEERAM NP, ZHANG YJ, LI L, GAO K. Strawberry consumption is associated with increased antioxidant capacity in serum[J].Journal of Medicinal Food,2010,13 (1) : 116-122.
[6] 左丽丽,王振宇,樊梓鸾,田双起.植物多酚类物质及其功能研究进展[J].中国林副特产,2012, (5):39-43.ZUO Lili,WANG Zhenyu,FAN Ziluan,TIAN Suangqi. Research Progress of Plant Polyphenols for Improving Cognitive Function[J]. Forest By-Productand Specialityin China ,2012, (5):39-43.
[7] CELESTINO SANTOS-BUSLGA. Recent advances in polyphenol research [M]. UK:Blackwell, 2010:269-282.
[8] JAN O, ANETA W. Polyphenols content and antoxidative activity in apple purées with rhubarbjuice supplement [J]. International Journal of Food Science and Technology, 2008, 43(3): 501-509.
[9] BEKTAS N, OZTURUK N. Antioxidant activity of Punica granatum (Pomegranate) flowers [J].Toxicology Letters,2007,(5):184-214.
[10] 廖霞,郑少杰,卢可可,肖星凝,吴素蕊,明建. 植物多酚通过Nrf2/ARE信号通路抗氧化作用研究进展[J].食品科学,2016, 37(7): 227-232.LIAO Xia, ZHENG Shaojie, LU Keke, XIAO Xingning, WU Surui, MING Jian. Plant Polyphenols Exert Antioxidant Activity of by Nrf2/ARE Signaling Pathway: A Review[J]. Food Science,2016, 37(7): 227-232.
[11] 伊娟娟,王振宇,曲航,李景彤.植物多酚抗肿瘤活性及其机制研究进展[J].食品工业科技,2016, 37(18):391-395.YI Juanjuan, WANG Zhenyu, QU Hang, LI Jingtong. Research progress in the anti-tumor activities and related mechanisms of plant polyphenols[J]. Science and Technology of Food Industry,2016, 37(18):391-395.
[12] 杨成涛,赵云令,孙云, 庄永亮.植物多酚抗骨质疏松作用的研究[J].食品工业科技,2014, 35(17):386-389.YANG Chengtao, ZHAO Yunling, SUN Yun, ZHUANG Yongliang. Effect of plant polyphenols on anti-osteoporosis[J]. Science and Technology of Food Industry, 2014, 35(17):386-389.
[13] 高航,徐虹.植物多酚抗肥胖作用研究进展[J].食品科学,2016, 37(18):334-338.GAO Hang, XU Hong. Progress in Understanding Anti-obesity Effects of Plant Polyphenols[J]. Food Science, 2016, 37(18):334-338.
[14] F GIAMPIERI, JM ALVAREZSUAREZ,SM BATTINO. Strawberry and human health: effects beyond antioxidant activity[J]. Journal of Agricultural and Food Chemistry,2014,62(18),3867-76.
[15] 张粹烂,张名位,廖森泰,魏振承,张雁,唐小俊,张瑞芬,邓媛元.荔枝果肉不同极性多酚提取及其抗氧化活性比较[J].华南师范大学学报,2011,(4):116-120.ZHANG Cuilan, ZHANG Mingwei, LIAO Sentai,WEI Zhencheng, ZHANG Yan,TANGXiaojun, ZHANG Ruifen, DENG Yuan-yuan. Comparison of the Content and Antioxidant Activities of Different Polarity Phenolics of Litchi Pulp[J].Journal of South China Normal Uniwersity,2011,(4):116-120.
[16] 热依木古丽.阿布都拉,刘力,阿吉艾克拜尔.艾萨.新疆石榴花多酚的提取工艺[J].食品科学,2011,32(2):158-161.Rahima ABDULLA, LIU Li, Haji Akber AISA1. Optimization of the Extraction Process ofPolyphenols from the Flower of Pomegranate (Punica granatum L.) in Xinjiang[J]. Food Science,2011,32(2):158-161.
[17] ZHANG Z, HUBER D J,SQU H, ,YUN Z, WANG H, HUANG Z, HUANG H, JIANG Y. Enzymatic browningand antioxidant activities in harvested litchi fruit as influenced by apple polyphenols [J].Food Chemistry,2015:191-199.
[18] 罗娅,唐勇,冯珊, 周迪,汤浩茹.6个草莓品种营养品质与抗氧化能力研究[J].食品科学,2011,32(7):52-56.LUO Ya, TANG Yong, FENG Shan, ZHOU Di , TANG Haoru. Comparison on NutritionalQuality and Antioxidant Capacity of Six Different Strawberry Cultivars[J]. Food Science,2011,32(7):52-56.
[19] 金亮,阮松林,忻雅,方献平,李小白,马华升,祝水金.森林草莓与栽培草莓体外抗氧化能力比较[J].核农学报,2015,29(1):52-56.JIN Liang, RUAN Songlin, XIN Ya, FANG Xianping, LI Xiaobai, MA Huasheng, ZHUShuijin. Comparison on Antioxidant Capacity in vitro of Fragaria vesca and StrawberryCultivars[J]. Acta Agriculturae Nucleatae Sinica,2015,29(1):52-56.
[20] GIAMPIERI F, ALVAREZ-SUAREZ JM, MAZZONI L. Strawberry enriched diet counteractsoxidative stress in plasma and liver of old rats and improves mitochondrial functionality [J]. FreeRadical Biology and Medicine, 2013, 65(2): S108.
[21] GIAMPIERI F, ALVAREZ-SUAREZ JM, TULIPANI S. Photoprotective potential of strawberry (Fragaria× ananassa) extract against UV-A irradiation damage on human fibroblasts [J]. J Agric Food Chem, 2012, 60(9): 2322-2327.
[22]侯玉茹,李文生,王宝刚, 苗飞,石磊.不同品种草莓抗氧化活性及品质研究[J].食品工业,2015,36(3):65-68.Hou Yuru,Li Wensheng, Wang Baogang, Miao Fei, Shi Lei. Study on Antioxidant Activityand Quality of Different Cultivars of Strawberries[J]. Food Industry,2015,36(3):65-68.
[23] 于漫漫,弓志青,曹晖, 王文亮,孙若琳,张璐.不同品种草莓抗氧化能力分析[J].中国食物与营养,2016,22(2):63-66.YU Manman, GONG Zhiqing, CAO Hui, WANG Wenliang,SUN Ruolin, ZHANG Lu.Analysis on Antioxidant Capacity of Different Varieties of Strawberries[J]. Food and Nutrition inChina,2016,22(2):63-66.
[24] 张毛莉,罗仓学. 石榴皮中总酚含量测定方法的比较[J].食品工业科技, 2011,32(5):383-388.ZHANG Maoli,LUO Cangxue. Study on determination of total polyphenolic compoundsin the pomegranate husk [J]. Science and Technology of Food Industry,2011,32(5): 383-388.
[25] SLINKARD K, SINGLETON V L. Total phenol analyses: automation and comparison with manualmethods [J] . American Journal of Enology and Viticulture, 1997, 28: 49-55.
[26] 涂斌,李新莹,姚舜,曹宇,吴子波,宋航. 甘蔗中原花青素含量的分析方法[J].食品研究与开发, 2009, 30(6): 130-133.TU Bin, LI Xinying, YAO Shun, CAO Yu, WU Zibo, SONG Hang. Analysis Methods of theContents of Procyanidins in Sugarcane[J]. Food Research and Development, 2009, 30(6):130-133.
[27] 蒋晓岚,孟菲,刘亚军,万根文,吴珂,夏涛,高丽萍. 茶树根原花青素提取工艺及检测方法的优化[J].安徽农业大学学报, 2013,40(6):891-898.JIANG Xiaolan, MENG Fei, LIU Yajun, WAN Genwen, WU Ke, XIA Tao, GAO Liping.Optimization of extraction technology and detection method on proanthocyanidins in tea root[J].Journal of Anhui Agricultural University, 2013,40(6):891-898.
[28] 王毕妮.红枣多酚的种类及抗氧化活性研究[D].杨陵:西北农林科技大学,2011.WANG Bi-ni. Kinds of Phenolic Compounds in Jujube and Antioxidant Acitivity[D].Yangli: North West Agriculture and Forestry University,2011
[29] 杨冬梅,金月亭,柯乐芹, 姜健美,黄晓丹,应铁进. 12种常见蔬菜抗氧化活性的比较研究[J].中国食品学报, 2007, 7(5): 24-29.Yang Dongmei, Jin Yueting, Ke Leqin, Jiang Jianmei, Huang Xiaodan, Ying Tiejin.Antioxidant Activities of 12 Common Vegetables[J]. Journal of Chinese Institute of Food Scienceand Technology,2007, 7(5): 24-29.
[30] 杜荣茂,杨虎清.成熟度对草莓果实抗氧化能力的影响[J].安徽农业科学,2013,41(4): 6466-6467.DU Rongmao, YANG Huqing. Effect of Maturity on Antioxidant Activities of Strawberry Fruit[J]. Journal of Anhui Agricultural Sciences,2013,41(4): 6466-6467.
[31] FRANCO C, JESSICA S, BRUNO M, MAURIZIO B. Combining quality and antioxidant attributes in the strawberry: the role of genotype[J]. Food Chemistry, 2008, 111: 872-878.
[32] RICARDO M F, SONIA Z V, ALICIA M, ALICIA R C. Growth and ripening season effects on antioxidant capacity of strawberry cultivar Selva[J].Scientia Horticulturae, 2007, 112: 27-32.
[33] FRANKEL E N. Antioxidants in lipid foods and food quality. Food Chemistry, 2008, 57: 51-55.
[34] 公丽艳,孟宪军,刘乃桥,毕金峰.基于主成分与聚类分析的苹果加工品质评价[J].农业工程学报,2014,30(13):276-285.Gong Liyan, Meng Xianjun, Liu Naiqiao, Bi Jinfeng. Evaluation of apple quality based on principal component and hierarchical cluster analysis[J]. Transactions of the Chinese Society of Agricultural Engineering,2014,30(13):276-285.
[35] 刘科鹏,黄春辉,冷建华,陈葵,严玉平,辜青青,徐小彪.‘金魁’猕猴桃果实品质的主成分分析与综合评价[J].果树学报,2012,29(5):867-871.LIU Kepeng, HUANG Chunhui, LENG Jianhua, CHEN Kui, YAN Yuping, GU Qingqing, XU Xiaobiao. Principal component analysis and comprehensive evaluation of the fruitquality of ‘Jinkui’ kiwifruit[J]. Journal of Fruit Science,2012,29(5):867-871.