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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (17): 121-124.

所属专题： 烟草种植与生产

• 农学 农业基础科学 • 上一篇    下一篇

化学发光法测定烟叶中的总氮

徐宸 孙家利 张凤侠 王卫武 祖朝龙 时向东   

  • 收稿日期:2009-03-13 修回日期:2010-04-29 出版日期:2010-09-05 发布日期:2010-09-05
  • 基金资助:

    国家烟草专卖局科技项目“皖南特色烟叶形成机理及配套技术研究”;江西省自然科学基金

Determination of the Content of Total Nitrogen in Tobacco Leaveswith Chemiluminescence Method

  • Received:2009-03-13 Revised:2010-04-29 Online:2010-09-05 Published:2010-09-05

PDF (PC)

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摘要：

建立测定烟叶中总氮含量的新方法。烟叶中的含氮化合物经消化后，加碱蒸馏，用硫酸吸收，全部转化为氨态氮，根据NH4+在碱性溶液中能还原ClO ，该反应和化学发光反应ClO?-－Luminol相耦合，降低化学发光强度，从而达到测定NH4+的目的。本方法的检测下限达到10-3mg/kg，线性范围选择0.01～1.5（mg/kg），加标回收率在87.3%～109.9%之间，RSD＜5%(n=5)，完全能满足烟叶中总氮测定的要求。

关键词: 文心兰, 文心兰, 原球茎, 增殖

Abstract:

A new method was established for determining the content of total nitrogen in tobacco leaves. After digested, stilled with alkali and absorbed by sulphuric acid, all the compounds which contain nitrogen in tobacco leaves change to NH4+. NH4+ can reduce ClO- in alkaline solution. This reaction couples with chemiluminescence reaction ClO-－Luminol, which reduce the strength of luminosity. So that the content of the NH4+is determined. The lower limit of detection reaches to 10-3 mg/kg. The choice of the line range is from 0.01 mg/kg to 1.5 mg/kg. The recovery of the method was 87.3% and 109.9%. And the RSD is less than 5%(n=5). These conditions are completely contented with the requirement of determination of the total nitrogen in tobacco leaves.