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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (17): 187-190.

所属专题： 园艺

• 农学 农业基础科学 • 上一篇    下一篇

立枯丝核菌侵染下AMF对黄瓜内源激素的影响

贺忠群 李焕秀 汤浩茹   

  • 收稿日期:2010-03-04 修回日期:2010-05-26 出版日期:2010-09-05 发布日期:2010-09-05
  • 基金资助:

    四川省教育厅重点项目

Effect of Arbuscular Mycorrhizal Fungi on Endogenous in Cucumber after Rhizoctonia solani Inoculation

  • Received:2010-03-04 Revised:2010-05-26 Online:2010-09-05 Published:2010-09-05

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摘要：

【研究目的】研究接种丛枝菌根真菌Glomus versiforme(GV)对接种立枯丝核菌黄瓜生长及根系内源激素的影响。【方法】采用盆栽试验，以不接种植株为对照对接种GV的植株接种立枯丝核菌，用酶联免疫法测定不同处理根系IAA、GA3、Zeatin、ABA含量，观测其动态变化。【结果】（1）GV与立枯丝核菌对黄瓜根系内源激素的影响不同。接种GV使黄瓜根系IAA、GA3、Zeatin、ABA含量增加，立枯丝核菌侵染使黄瓜IAA、ABA含量增加，而GA3和Zeatin含量下降。（2）与对照病株相比，接种GV植株根系具有较高IAA、Zeatin、GA3含量而ABA含量较低。【结论】接种GV能促进植株生长，接种GV植株根系IAA、GA3、Zeatin含量相对增加和增强植株的抗病能力有关，IAA在植株对病菌侵染的防御过程中具有一定的作用。

关键词: 催乳素受体基因, 催乳素受体基因, 繁殖性能, 遗传多态性, PCR—SSCP, PCR—RFLP

Abstract:

【Objective】The purpose was to study the effect of arbuscular mycorrhizal fungi on plant growth and endogenous in cucumber Rhizoctonia solani .【Method】 In pot culture, the plant with no inoculation as control, cucumber infected by GV was inoculated with Rhizoctonia solani and dynamic variation of IAA、GA3、Zeatin、ABA in cucumber roots in different treatments were decided by the method of ELIA.【Results】(1) The effect of GV and Rhizoctonia solani on endogenous in cucumber root was different. AMF inoculation improved the content of IAA、GA3、Zeatin、ABA. these endogenous hormones, whereas Rhizoctonia solani inoculation improved the content of IAA、ABA and decreased GA3、Zeatin at the same time. (2)Higher content of IAA、GA3、Zeatin in root inoculated with GV and lower ABA was detected compared to the control infected by Rhizoctonia solani.【Conclusion】GV improved plant growth and the improved disease tolerance was related to the content of IAA、GA3、Zeatin induced by GV. IAA has certain role in plant defense against pathogen infection process