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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (17): 26-30.

所属专题： 食用菌； 食用菌

• 生物技术科学 • 上一篇    下一篇

平菇、杏鲍菇和白灵菇菌丝多糖对-OH、DPPH-和NO2-的体外清除作用

刘海英 张运峰 范永山 刘青   

  • 收稿日期:2010-03-23 修回日期:2010-04-29 出版日期:2010-09-05 发布日期:2010-09-05
  • 基金资助:

    河北省科技支撑计划项目:

In vitro clearance of -OH、DPPH- and NO2- with polysaccharides from mycelia of Pleurotus ostreatus, P. nebrodensis and P. eryngii

  • Received:2010-03-23 Revised:2010-04-29 Online:2010-09-05 Published:2010-09-05

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摘要：

【研究目的】明确平菇、白灵菇和杏鲍菇菌丝多糖对-OH、DPPH-和NO2-自由基的清除能力；【方法】利用热水浸提法提取菌丝多糖，Sevag法纯化多糖，利用多功能酶标仪测定对-OH、DPPH-(1,1-diphenyl-2-picrylhydrazyl)、NO2-的清除率，利用DPS软件进行统计分析；【结果】在200-2000 μg/mL浓度范围内，白灵菇B10、杏鲍菇PL7和平菇P89菌丝多糖对DPPH-的清除率为5.98％-51.96％，EC50值分别为1.98、2.74和3.28mg/mL；对-OH清除率为43.51％-98.09％，PL7的EC50值溢出最小值，B10和P89的EC50值分别为49.6μg/mL和467.3 μg/mL；对NO2-的清除率为10.22％-26.75％，P89的EC50值为5.3mg/mL，PL7和B10的菌丝多糖浓度高于500μg/mL时，清除率不再增加，最高值分别为18.53%和17.52%。【结论】菌种间抗氧化能力差异明显，同一菌株对不同自由基的清除能力也存在显著差异。3种供试菌种多糖具有较强的清除-OH能力，中等的清除DPPH-能力和较差的清除NO2-能力。杏鲍菇PL7和白灵菇B10菌丝多糖的清除-OH和DPPH-能力较强，而平菇P89具有较强的清除NO2-能力。

关键词: 小麦, 小麦, 条锈病, 抗性基因, 分子标记

Abstract:

【OBJECTIVE】This paper is to clearify the in vitro clearance ability of -OH, DPPH- and NO2- with the mycelial polysaccharides from Pleurotus ostreatus, P. nebrodensis and P. eryngii;【METHOD】Mycelial polysaccharides were extracted by hot water and purified by Sevag method. The anti-oxidation activity was determined by polyfunctional microplate spectrophotometer. The data were analyzed by DPS V7.05 software;【RESULTS】. The clearance rates of -OH, DPPH- and NO2- were 43.51％-98.09％, 5.98％-51.96％ and 10.22％-26.75％ respectively in the polysaccharide concentration range of 200-2000 μg/mL. The EC50 values of DPPH- clearance rate for B10, PL7 and P89 are 1.98, 2.74 and 3.28mg/mL respectively. The EC50 values of -OH clearance rate for P89 and B10 are 49.6μg/mL and 467.3 μg/mL respectively. The EC50 value of -OH clearance rate for PL7 is beyond the minimum limit. The EC50 value of NO2- clearance rate for P89 is 5.3mg/mL; The maxima of NO2- clearance rate for PL7 and B10 are 18.53% and 17.52% respectively when the polysaccharide concentration is above 500μg/mL,【CONCLUSION】The anti-oxidation activity is different between mushrooms and for different free radicals. The tested mushrooms have high ability to clear -OH, middle ability to clear DPPH- but weak ability to clear NO2-. PL7 and B10 have high clearance ability to-OH and DPPH-, but P89 is at the advantage of NO2- clearance ability.