欢迎访问《中国农学通报》,

中国农学通报 ›› 2010, Vol. 26 ›› Issue (17): 194-196.

所属专题: 生物技术 油料作物 园艺

• 农学 农业基础科学 • 上一篇    下一篇

几种化学物质对油菜叶片β-1,3-葡聚糖酶活性诱导的研究

李伟 陈伟 熊涛   

  • 收稿日期:2010-03-25 修回日期:2010-04-21 出版日期:2010-09-05 发布日期:2010-09-05
  • 基金资助:

    湖北省教育厅优秀中青年基金项目;长江大学科研发展基金项目

Inducing β-1, 3-Glucanase Activities of Rape by Four Chemical Inducers

  • Received:2010-03-25 Revised:2010-04-21 Online:2010-09-05 Published:2010-09-05

摘要:

摘要:【研究目的】在温室内种植中油杂2号油菜,当长至2-3片真叶时,使用水杨酸(10mg/mL)、核黄素(1mmol/L)、氯化汞(0.01%)和BTH(0.5mmol/L)四种化学物质,对叶片进行诱导处理,研究其对油菜叶片β-1,3-葡聚糖酶的诱导。【结果】水杨酸、核黄素和氯化汞处理的油菜叶片,β-1,3-葡聚糖酶在短期内活性达到第一个高峰后随之下降,然后又上升。BTH处理的油菜叶片在处理后第五天达到活性高峰。【结论】几种化学物质处理后油菜叶片中β-1,3-葡聚糖酶的活性均有不同程度的提高;其中以BTH的诱导作用最强。对于油菜β-1,3-葡聚糖酶活性诱导的研究有利于深入了解β-1,3-葡聚糖酶在病程反应过程中的作用。

关键词: 玉米, 玉米, 弘大8号, 宽窄行, 密度

Abstract:

In the greenhouse, rape leaves were sprayed with four chemical inducers (0.01% mercuric chloride, 10 mg/mL salicylic acid, 1 mmol/L riboflavin, 0.1 mmol/L BTH, respectively) from 2008 to 2009. The leaves treated with water as control. Then, all the leaves were collected and used to extract the β-1, 3-glucanase and to measure the activities. When treated with mercuric chloride, salicylic acid, riboflavin, the β-1, 3-glucanase activity raised in the begin days and declined. However, the β-1, 3-glucanase activity raised to the peak on the six day when treated with BTH. The results showed that the levels of β-1, 3-glucanase activity were all induced when treated with four chemical inducers. Moreover, the BTH has the best effect on induction of the β-1, 3-glucanase.