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中国农学通报 ›› 2010, Vol. 26 ›› Issue (17): 47-50.

所属专题: 生物技术 油料作物

• 生物技术科学 • 上一篇    下一篇

花生AhLEA18蛋白基因的克隆与表达分析

苏磊 赵传志 王兴军 毕玉平   

  • 收稿日期:2010-05-17 修回日期:2010-06-11 出版日期:2010-09-05 发布日期:2010-09-05
  • 基金资助:

    科技部863计划重大项目“花生脂肪酸代谢相关基因的分离与功能研究”;山东省农科院院创新基金项目“花生籽粒全长cDNA文库的大规模测序及脂肪酸合成关键酶基因分离和功能分析”;山东省农科院院创新基金项目“花生品质(脂肪酸、蛋白质)相关功能基因挖掘及高效转基因技术体系能研究”;山东省自然科学基金项目“抑制PEPase基因表达提高花生油份含量的研究”

Cloning and Expression Analysis of Peanut AhLEA18

  • Received:2010-05-17 Revised:2010-06-11 Online:2010-09-05 Published:2010-09-05

摘要:

LEA蛋白家族是一类在种子胚胎发育晚期丰富表达的蛋白,在种子的正常发育过程中大量积累,同时在植物处于干旱、寒冷、盐胁迫等逆境条件下高水平表达,推测该类蛋白在缺水条件下具有一定的生理保护功能。根据蛋白序列特点,该蛋白家族分为多个组,LEA18蛋白属于其中一组蛋白。PvLEA18蛋白在干燥种子、花粉粒中大量积累,也参与到对水分缺失、ABA处理的生理应答过程过程。本研究从栽培种花生鲁花14中克隆得到一个LEA18基因,命名为AhLEA18。对其序列的生物信息学分析表明,AhLEA18具有典型LEA18蛋白序列特征。通过半定量RT-PCR的方法对该基因在花生植株不同组织及不同发育时期的种子中的表达情况进行了分析。

关键词: 鲢鳙鱼, 鲢鳙鱼, 藻类, 蠡湖

Abstract:

In plants, a group of hydrophilic proteins, known as Late Embryogenesis Abundant (LEA) proteins, accumulate to high level during the late stage of seed maturation and during water deficiency in vegetative organs, suggesting a protective role during water limitation. LEA18 protein is a member of LEA protein family. The PvLEA18 transcript and protein level are highly accumulated in dry seeds and pollen grains and also respond to water deficit and ABA treatments. In this study, one LEA18 protein gene from peanut (LuHua 14) was cloned and named AhLEA18. Aligning with several LEA18 proteins from other species by ClustalW program, the result demonstrated that the AhLEA18 is a typical LEA18 protein. The expression of this gene in different tissues and four developmental stages of peanut seeds were analyzed by Semi-quantitative RT-PCR.

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