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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (17): 9-12.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

基于连接酶检测反应的NPY基因多态与清远麻鸡繁殖性状的相关性研究

张莹 束婧婷 韩威 宋卫涛 陈宽维 李慧芳   

  • 收稿日期:2010-05-20 修回日期:2010-06-05 出版日期:2010-09-05 发布日期:2010-09-05
  • 基金资助:

    现代农业产业技术体系建设专项资金;江苏省自然科学基金;江苏省家禽科学研究所青年（博士）基金

A study on the relativity between NPY gene polymorphism and reproductive traits of Qingyuan partridge chicken based on ligase detection reaction

  • Received:2010-05-20 Revised:2010-06-05 Online:2010-09-05 Published:2010-09-05

PDF (PC)

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摘要：

基因多态性蕴藏着大量遗传信息，分型技术是研究多态性的主要研究手段。本文建立了一个基于聚合酶链反应—连接酶检测反应的基因多态性检测系统，探讨清远麻鸡神经肽Y基因（NPY）的多态性，并分析其对清远麻鸡开产日龄、开产蛋重、开产体重、40周平均蛋重、50周平均蛋重以及52周产蛋数的遗传效应。对NPY基因转录起始去4 bp的缺失突变进行基因型分型，结果同直接测序分型结果一致。不同基因型个体间仅在开产体重上存在显著差异，BB型个体的开产体重显著低于AA和AB型个体。

关键词: 关键词:安定区, 关键词:安定区, 退耕还林, 生态效益, 经济价值

Abstract:

Gene polymorphisms include lots of genetic information. Typing technology is the main method to evaluate gene polymorphism. The parallel typing system based on ligase detection reaction was established in the present study, and to detect the polymorphism of NPY gene, as well as its genetic effect on age at first laying, egg weight at first laying, body weight at first laying, average egg weight at the age of 40 weeks, average egg weight at the age of 50 weeks, and total egg number at the age of 52 weeks in Qingyuan partridge chicken. The genotypes of 4 bp deletion mutation in NPY transcription start region were identified in Qingyuan partridge chickens, and the results were coincidence with direct sequencing. The least square analysis showed that only body weight at first laying has significantly difference between different genotypes. BB genotype birds had significant lighter body weight at first laying than AA and AB genotype birds