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中国农学通报 ›› 2010, Vol. 26 ›› Issue (17): 60-64.

• 生物技术科学 • 上一篇    下一篇

掌叶木种子的离体培养与玻璃化逆转研究

李在留 郭松 陈晴 胡佩龙 乔梦吉 和太平   

  • 收稿日期:2010-06-24 修回日期:2010-07-08 出版日期:2010-09-05 发布日期:2010-09-05
  • 基金资助:

    广西区林业厅科技项目;广西大学校基金项目

Study on Culture in Vitro and Reversion for Vitrification on Seeds of Handeliodendron bodinieri (Lévl.) Rehd.

  • Received:2010-06-24 Revised:2010-07-08 Online:2010-09-05 Published:2010-09-05

摘要:

为探索国家Ⅰ级重点保护野生植物掌叶木的快速繁殖技术,采用离体培养法,以掌叶木种子为外植体,在含有不同生长调节剂的培养基上诱导萌芽及丛芽。结果表明:在适量的生长调节剂作用下,掌叶木种子现采现培养发芽率可达96.7%,随着贮藏时间的延长,发芽率逐渐降低,半年后全部失去发芽力。BA有利于苗的增粗生长,NAA有利于苗的高生长。采用MS+BA1.0 mg/L+NAA0.2 mg/L培养基可诱导产生生长状态良好,增殖倍数为3.43的丛芽,但随着继代次数的增加,易产生玻璃化现象。将MS培养基换为B5培养基,BA-NAA组合换为KT-IBA组合,可以有效逆转玻璃化苗。正交试验结果表明:KT的含量对丛芽增殖倍数和玻璃化逆转起决定作用,其次是培养基,采用0.75B5+KT1.0 mg/L+IBA0.05 mg/L+蔗糖40000 mg/L培养基配方可以获得玻璃化逆转率和增殖率较高的丛芽。

关键词: 空气凤梨, 空气凤梨, 特征, 室内装饰, 研究进展

Abstract:

The method of culture in vitro was applied to study rapid propagation of Handeliodendron bodinieri (Lévl.) Rehd. which is an endangered wildlife catalogued as a national protected plant of China (Category I). Seeds of H. bodinieri were used as explants to induce sprouts and buds on medium contained different growth regulators. The results indicated that with suitable growth regulators, if seeds of H. bodinieri were collected and cultured in time germination rate could reach 96.7%. But with storage duration, the germination rate cut down gradually and wouldn’t have the ability of germination after half a year. The BA could make plants stronger and NAA could make plants taller. Buds grew well and the proliferation multiple was 3.43 on the medium of MS+BA1.0 mg/L+NAA0.2 mg/L. But with subcultures increasing, vitrification emerged. Vitrification could be reversed efficiently with changing MS medium into B5 medium and BA-NAA group into KT-IBA group. Orthogonal experiments results showed that the proliferation multiple and reversion for vitrification of buds were determined by the content of KT and medium was the second factor. The compounding medium of 0.75B5+KT1.0 mg/L+IBA0.05 mg/L+Sucrose 40000 mg/L could induce buds with high proliferation multiple and reversion of vitrification.