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中国农学通报 ›› 2010, Vol. 26 ›› Issue (17): 37-40.

所属专题: 生物技术 畜牧兽医

• 生物技术科学 • 上一篇    下一篇

偏凸-柱穗山羊草双二倍体SDAU18 PMC MI染色体数目异常

王玉海 郑美 刘晓松 王洪刚   

  • 收稿日期:2010-07-07 修回日期:2010-07-22 出版日期:2010-09-05 发布日期:2010-09-05

Abnormal Chromosome Number in PMC MI of Aegilops ventricosa × Aegilops cylindrica Amphiploid SDAU18

  • Received:2010-07-07 Revised:2010-07-22 Online:2010-09-05 Published:2010-09-05

摘要:

摘要:为探讨偏凸-柱穗山羊草双二倍体SDAU18减数第一分裂中期(PMC MI)染色体丢失特点。利用改良卡宝品红压片法,对SDAU18及其双亲的PMC MI染色体构型进行观察。结果表明:在SDAU18 的同一枚花药中,部分PMCs MI出现了不同程度的染色体丢失现象,发生染色体丢失的PMC MI频率平均为4.64%,染色体数目异常的PMCs MI的平均染色体构型为:2n = 12.77 I + 5.26 IIR + 1. 57IIO + 0. 02III = 26.49。其双亲的PMC MI的染色体数目均表现正常,均由14个二价体组成。SDAU18 PMCS MI染色体数目变异的特点比较罕见。

关键词: 小白鼠, 小白鼠, 腹泻模型, 小肠粘膜, 自愈, 病理变化

Abstract:

Abstract: To study the chromosome elimination of PMC MI in amphiploid SDAU18 from Ae. ventricosa and Ae. cylindrica. The chromosome configuration of SDAU18 and its parents was observed by improved carbol fuchsin methods. The results indicated that chromosome loss of ranged number in partial PMCs MI in one anther of SDAU18 was observed. The average frequency of chromosome-lost PMC MI was 4.64%, and the mean configuration of chromosome-lost PMCs MI was 2n = 12. 77I + 5.26IIR + 1. 57IIO + 0. 02III = 26.49。. However, PMCs MI with abnormal number in its parents weren’t found, each cell had 14 bivalents. The chromosme elimination of SDAU18 PMCs MI was rare.