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刘杨1,张海予2,韩涛2.扩展青霉拮抗菌的筛选鉴定及其发酵液的抑菌效果[J].中国农学通报,2010,26(5):8-13..The screening and identification of antagonistic bacteria against Penicillium expansum and the inhibitive effects of fermentation broth[J].Chinese Agricultural Science Bulletin,2010,26(5):8-13
扩展青霉拮抗菌的筛选鉴定及其发酵液的抑菌效果
The screening and identification of antagonistic bacteria against Penicillium expansum and the inhibitive effects of fermentation broth
投稿时间:2009-10-27  修订日期:2009-11-13
DOI:
中文关键词: 

抑菌活性


英文关键词: 

inhibitive activity


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刘杨1  张海予2  韩涛2
1北京农学院植物科学与技术学院,北京102206;2北京农学院食品系,北京102206
中文摘要:
      

从水果表面及土壤中分离到几十株细菌,通过平板筛选到6株拮抗效果较好的拮抗菌,其中2株拮抗菌T3和T8的发酵液对扩展青霉(Penicillium expansum)表现出显著的拮抗效果。本实验对该两种菌的发酵液的抑菌作用进行了初步的研究。这两株拮抗菌T3和T8的发酵液稳定性表现出不同,分别用100℃高温或两种蛋白酶处理后,T3菌株的发酵液抑菌效果均有一定程度的下降但仍表现出较高的活性,而T8菌株的发酵液在100℃高温处理后,抑菌活性下降但仍保持部分活性,但用蛋白酶处理,其抗菌活性几乎丧失。T3与T8发酵液的抑菌活性在培养2天后几乎达到最大,T3的最适发酵培养基为BPA,T8的最适发酵培养基为PDA。两株菌的发酵液处理扩展青霉菌的菌丝后,引起菌丝畸形,原生质凝缩,出现空泡。根据细菌16SrDNA序列分析及其形态特征和生理生化特性,初步鉴定T3和 T8均为枯草芽孢杆菌(Bacillus subtilis),两株菌之间基因序列的相似性为93.45%。

英文摘要:
      

Dozens of strains of bacteria were isolated from the surface of fruits and the soil, they were selected through the plate confrontation method and six strains which have good antagonistic effect are obtained, among them two strains named T3 and T8 which the fermentation broth showed a significant antagonistic effect to Penicillium expansum are found. The antifungal activity of fermentation broth of T3 and T8 are studied. The stability experiment show that after treatment with 100 ℃ and two proteases, the fermentation broth of T3 performs good antagonistic effect, while antagonistic effect of the fermentation broth of T8 decreases a lot. The antifungal activity of T3 and T8 reach almost highest level after 2 days growth. It is also found that BPA was the optical medium to T3 and PDA was the optical medium to T8. The fermentation broth of two strains can cause hyphae deformity of Penicillium expansum. According to their morphological, physiological, biochemical characters, and the analysis of the sequence of 16SrDNA,T3 and T8 were preliminary identified as Bacillus subtilis, two strains of the similarity between the gene sequence is 93.45%.

作者简介:张海予
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