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梁锐婷,魏丽娜,接伟光,李亮,蔡柏岩,等.摩西管柄囊霉Funneliformis mosseae对连作大豆苗期根系AM真菌菌群结构的影响[J].中国农学通报,2018,34(1):18-26.,et al.Effect of Funneliformis mosseae on Community Structure of Arbuscular Mycorrhizal Fungi of Soybean Root at Seedling Stage in Continuous Cropping Soil[J].Chinese Agricultural Science Bulletin,2018,34(1):18-26
摩西管柄囊霉Funneliformis mosseae对连作大豆苗期根系AM真菌菌群结构的影响
Effect of Funneliformis mosseae on Community Structure of Arbuscular Mycorrhizal Fungi of Soybean Root at Seedling Stage in Continuous Cropping Soil
投稿时间:2016-11-12  修订日期:2017-12-14
DOI:10.11924/j.issn.1000-6850.casb16110058
中文关键词: 连作大豆  苗期  摩西管柄囊霉  AM真菌菌群结构  Nested-PCR-DGGE
英文关键词: continuous cropping soybean  seedling stage  Funneliformis mosseae  community structure of arbuscular mycorrhizal fungi  Nested-PCR-DGGE
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基金项目:国家自然科学基金面上项目,摩西管柄囊霉(Funneliformis mosseae)抑制连作大豆根腐病发病机理的研究(31570487);黑龙江东方学院横向科研课题重点项目“摩西管柄囊霉修复连作大豆根际土壤微环境的机制”(HDFHX160103)。
作者单位E-mail
梁锐婷 黑龙江大学 生命科学学院 26786075@qq.com 
魏丽娜 黑龙江东方学院 食品与环境工程学部 737320321@qq.com 
接伟光 黑龙江东方学院 食品与环境工程学部 jieweiguang2007@126.com 
李亮 黑龙江大学 生命科学学院 399754613@qq.com 
蔡柏岩 黑龙江大学 生命科学学院 caibaiyan@126.com 
中文摘要:
      中国北方大豆连作问题日益突出并受到广泛关注,为了将AM真菌应用于连作土壤,缓解大豆连作障碍。本试验采用Nested-PCR-DGGE技术分析接种摩西管柄囊霉(Funneliformis mosseae)对3种连作年限3种品种大豆苗期根系AM真菌菌群结构的影响。结果表明,接种F. mosseae后,同一品种连作两年大豆根系中AM真菌的侵染率最高,同一连作年限KF16根系中AM真菌的侵染率最高;不同连作年限不同品种大豆根系中AM真菌菌群中均含有粘性球囊霉(Glomus viscosum)和未培养的球囊霉属(Uncultured Glomus);种植于连作2年土壤的不同品种大豆根系AM真菌的丰度值和Shannon-weine指数均高于连作0年和连作1年的。本研究揭示接种F. mosseae对连作1年、连作2年不同品种大豆苗期根系AM真菌菌群结构影响显著。
英文摘要:
      Continuous cropping of soybean (Glycine max) in north China is an increasingly prominent problem and attracting widespread attention. Arbuscular mycorrhizal fungi (AMF) are applied in continuous cropping soil in order to reduce continuous cropping obstacles. In this study, Nested-PCR-DGGE technology was used to analyze the effect of Funneliformis mosseae on community structure of AMF in three different continuous cropping soils for three soybean varieties at seedling stage. The results indicated that the infection rate of AMF was the highest in the same soybean variety with two continuous cropping years after inoculating F. mosseae. The colonization rate of AMF was the maximum in KF16 in the same continuous cropping year. There were Glomus viscosum and Uncultured Glomus in the soybean varieties’roots at different continuous cropping years. The abundance value and Shannon-Wiener index of AMF of soybean roots with two continuous cropping years were higher than those with zero continuous cropping year and one continuous cropping year. It revealed that F. mosseae significantly influenced the community structure of AMF in different soybean varieties’roots of one continuous cropping year and two continuous cropping years at seedling stage.
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