• 首页期刊简介编委会投稿须知论文要求/规范
梁乃国,程大友.吲哚-3-乙酸(IAA)和脱落酸(ABA)在甜菜生长发育过程中的分布[J].中国农学通报,2018,34(19):5-11..Distribution of Indole-3-acetic acid (IAA) and Abscisic Acid (ABA) in the Development of Beta vulgaris[J].Chinese Agricultural Science Bulletin,2018,34(19):5-11
吲哚-3-乙酸(IAA)和脱落酸(ABA)在甜菜生长发育过程中的分布
Distribution of Indole-3-acetic acid (IAA) and Abscisic Acid (ABA) in the Development of Beta vulgaris
投稿时间:2017-11-02  修订日期:2018-01-23
DOI:10.11924/j.issn.1000-6850.casb17110007
中文关键词: IAA,ABA,组织,UPLC/MS/MS
英文关键词: IAA, ABA, tissues, UPLC/MS/MS
摘要点击次数: 299
全文下载次数: 100
基金项目:国家自然科学基金“甜菜Owen 型细胞质雄性不育恢复基因(Rf)的克隆及PPR 基因家族的研究(31271781)”,“甜菜新型雄性不育细胞质 (K-5)线粒体基因结构及不育机制研究”(31371685)。
作者单位E-mail
梁乃国 哈尔滨工业大学化工与化学学院 naiguoliang@163.com 
程大友 哈尔滨工业大学化工与化学学院 hgdcdy@126.com 
中文摘要:
      本研究旨在明确IAA和ABA在甜菜发育过程中的作用机制。用生物信息学方法鉴定出IAA和ABA生物合成酶基因,通过qRT-PCR检测基因的表达并结合超高效液相色谱质谱(UPLC/MS/MS)定量 分析IAA 和ABA的浓度变化。结果表明BvIAA6、BvIAA26 和BvIAA33 基因在甜菜茎和花蕾中协同调节IAA 激素含量的变化,BvABA2、BvABA3、BvNCED1 和BvNCED5 基因调控ABA 的含量变化;研究发现ABA的含量高于IAA,但是IAA在促进植物生长方面的作用强于ABA;IAA和ABA生物合成酶基因具有组织特异性表达的特征。研究阐明了内源性IAA和ABA在甜菜生殖生长过程中的分子机制,为作物的分子育种提供了应用性参考。
英文摘要:
      This study aims at clarifying the molecular mechanisms of IAA and ABA in the development of sugar beet. The IAA and ABA biosynthesis genes were identified by bioinformatics. The gene expression was detected by qRT-PCR, and the concentrations of IAA and ABA were analyzed quantitatively by UPLC/MS/MS. Results showed that BvIAA6, BvIAA26 and BvIAA33 coordinately regulated IAA concentration, while ABA concentration was controlled by BvABA2, BvABA3, BvNCED1 and BvNCED5. ABA concentration was higher than that of IAA, but IAA was more powerful to promote plant growth than ABA. IAA and ABA biosynthesis genes had tissue specific expression. These findings elaborate the molecular mechanism of IAA and ABA in reproductive growth of sugar beet and provide references for crop breeding.
作者简介:
查看全文   HTML   发表评论  下载PDF阅读器