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王希,陈丽,赵春雷,等.表型与分子标记相结合分析130份糖用甜菜种质的遗传多样性[J].中国农学通报,2018,34(30):26-36.,et al.Genetic Diversity Analysis of 130 Accessions Beta vulgaris Germplasms with Phenotype and Molecular Marker[J].Chinese Agricultural Science Bulletin,2018,34(30):26-36
表型与分子标记相结合分析130份糖用甜菜种质的遗传多样性
Genetic Diversity Analysis of 130 Accessions Beta vulgaris Germplasms with Phenotype and Molecular Marker
投稿时间:2018-05-07  修订日期:2018-09-30
DOI:10.11924/j.issn.1000-6850.casb18050020
中文关键词: 甜菜  遗传多样性  表型  SSR分子标记
英文关键词: Beta vulgaris (sugar beet)  genetic diversity  phenotype  simple sequence repeat (SSR) molecular marker
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基金项目:国家甜菜现代农业产业技术体系“甜菜种质资源评价”岗位(CARS-210101);国家现代农业产业技术体系(糖料)建设专项资金(Sugar Crop Research System, CARS)“甜菜种质资源收集与评价”(CARS-170102);2017 年度黑龙江省省属高等学校基本科研业务费科技创新类重点项目“甜菜 分子标记位点库的深度挖掘与候选SSR标记开发”(KJCXZD201713);黑龙江省教育厅科学技术研究面上项目“甜菜基因组中分子标记位点的挖掘” (12531488);黑龙江大学青年科学基金项目“甜菜基因组中SSR分子标记位点的挖掘”(QL201124)。
作者单位E-mail
王希 黑龙江大学农作物研究院 lagow@163.com 
陈丽 黑龙江大学农作物研究院 caaschenli@163.com 
赵春雷 黑龙江大学农作物研究院 zhaocl-1@126.com 
李彦丽 黑龙江大学农作物研究院 hdnxylyl@126.com 
中文摘要:
      旨在对甜菜种质进行比较系统的遗传多样性分析与遗传距离分析。将表型与分子标记相结合,从3 个角度分析了130 份甜菜种质的遗传多样性,并对种质进行了遗传距离的分析。130 份甜菜种质表现出比较丰富的遗传多样性,其中22 项描述性性状的多样性指数在0.0451~1.1867 之间,平均值为0.8559;23 项数值型性状的变异系数在2.94%~594.84%之间,平均值为59.10%;34 个SSR标记的多态性信息含量在0.1289~0.7847 之间,平均值为0.4887。联合使用形态标记与分子标记计算遗传距离,将130份种质分成了5个近缘组,相邻组间产糖量、块根锤度、根腐病病情指数3项性状均有显著差异。结果表明被分析的甜菜种质遗传多样性比较丰富,遗传距离分析结果有生物学意义,可为种质创新工作提供参考。
英文摘要:
      The aim is to study the genetic diversity and genetic distances of sugar beet germplasms. Phenotype and molecular markers were combined to analyze the genetic diversity of 130 accessions B. vulgaris germplasms from three aspects, and the genetic distances were also analyzed. 130 accessions B. vulgaris germplasms showed more abundant genetic diversity. The Shannon- wiener indexes of 22 descriptive characters were between 0.0451 and 1.1867 with an average of 0.8559. The coefficients of variation (CV ) of 23 numerical characters were from 2.94% to 594.84% with an average of 59.10%. The polymorphism information contents (PIC ) of 34 SSR markers were 0.1289-0.7847 with an average of 0.4887. Morphological markers and molecular markers were combined to calculate genetic distances, and these 130 germplasms were divided into five related groups. Sugar yield, brix of root and root rot disease index exhibited significant differences between all adjacent groups. These results suggest that the analyzed B. vulgaris germplasms have abundant genetic diversity, the genetic distance analysis has biological significance and can be the reference for germplasm innovation.
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