Abstract: In an effort to test scientific and reasonable bioassay of Thrips hawaiiensis, laboratory studies were conducted on the effect of three bioassay methods including centrifuge tube residual bioassay, leaf-dip bioassay and leaf-tube residual bioassay on toxicity of five original insecticides to against larvae of T. hawaiiensis. It was revealed that toxicity results of the 5 insecticides varied differently among the treatments, leaf-tube residual bioassay performed the highest toxicity and the toxicity (LC50) order was emamectin-benzoate(0.36 mg/L)＞ chlorpyrifos(0.90 mg/L)＞ imidacloprid(1.64 mg/L)＞ betacy-permethrin(4.70 mg/L)＞ buprofezin(12.53 mg/L), which was consistent with centrifuge tube residual bioassay, for emamectin-benzoate(1.63 mg/L)＞ chlorpyrifos(2.18 mg/L)＞ imidacloprid(6.59 mg/L)＞ betacy-permethrin(7.38 mg/L)＞ buprofezin(22.06 mg/L). Whereas toxicity order was imidacloprid (2.15 mg/L)＞ emamectin-benzoate(2.29 mg/L)＞ betacy-permethrin(7.21 mg/L)＞ chlorpyrifos(7.40 mg/L)＞ buprofezin(14.95 mg/L) when leaf-dip bioassay was applied in the research. In summary, present study suggested above three methods would be establish and apply in laboratory bioassay on Thrips hawaiiensis, and centrifuge tube residual bioassay was more suitable for using in contact toxicity bioassay.