关键词: 寄生蜂, 寄生蜂

Abstract: To learn the effect and mechanism of Chinese herbal compound prescription. In this study, LPS was used to establish endometrial epithelial cell model of cow inflammatory injury, 100 ?g/mL lipopolysaccharide was chosen to treat the epithelial cell for 12 h, which was screened by MTT assay. Chinese herbal compound prescription Gong Jing of 2 mg/mL was used to cure cow inflammatory injury. Then IL-1β, IL-6 and IL-8 mRNA was detected at 3h, 6 h and12 h respectively by Real-time-PCR. The results showed that compared with the control group, IL-1β, IL-6 and IL-8 was significantly increased at 6 h, 12 h of model group (P<0.01); IL-1β and IL-8 was significantly elevated at 3 h of model group (P<0.05). Compared with model group, Gong Jing made IL-1β, IL-6, IL-8 decrease significantly to 3h model group (P<0.05, P<0.01), while IL-6, IL-8 reduced notably to 6 h model group (P<0.01), and only IL-8 decreased to 12 h model group(P<0.05). In conclusion, LPS could induce IL-1β, IL-6, IL-8 mRNA expression, and traditional Chinese medicine had a certain anti-inflammatory effect by modulating the IL-1β, IL-6, IL-8 mRNA expression.