‘大白谷’ CC-NBS-LRR蛋白编码基因抗干尖线虫侵染时空表达研究

doi:10.11924/j.issn.1000-6850.casb15010080

中国农学通报 ›› 2015, Vol. 31 ›› Issue (21): 279-283.doi: 10.11924/j.issn.1000-6850.casb15010080

所属专题：生物技术

‘大白谷’ CC-NBS-LRR蛋白编码基因抗干尖线虫侵染时空表达研究

陈俏丽,王峰,李丹蕾,孔畅仪,牛春阳,张瑞芝,王博文,刘爽,史欣悦,张荣

东北林业大学,东北林业大学,东北林业大学,黑龙江省中医药大学,东北林业大学,东北林业大学,东北林业大学,东北林业大学,东北林业大学,东北林业大学

收稿日期:2015-01-14 修回日期:2015-06-26 接受日期:2015-02-25 出版日期:2015-07-28 发布日期:2015-07-28
通讯作者: 王峰
基金资助:
高等学校博士学科点专项科研基金资助课题（博士点新教师基金资助项目）“水稻干尖线虫诱导水稻防卫反应基因分析”(20100062120002)；大学生创新性实验计划项目“植物抗病基因研究”(201410225162)。

Spatial-temporal Expression of CC-NBS-LRR Protein-coding Gene Resisting to Infection of Aphelenchoides besseyi in Oryza sativa L. ssp. Indica

Received:2015-01-14 Revised:2015-06-26 Accepted:2015-02-25 Online:2015-07-28 Published:2015-07-28

摘要/Abstract

摘要： 为深入探究‘大白谷’ (Oryza sativa L. ssp. Indica)感病的分子机理，以及抗病基因在发病过程中所起的作用，克隆了‘大白谷’ CC-NBS-LRR蛋白编码基因 Os11gRGA8，对 Os11gRGA8基因进行了生物信息学分析，并对其在水稻抗干尖线虫侵染过程中的功能进行了研究。Os11gRGA8基因位于 11号染色体，其编码蛋白含有733个氨基酸，等电点为5.99，相对分子质量为84355.07。序列分析表明该氨基酸序列含有 RX-CC、 AAA_22、 NBS 和 LRR_4 这 4 个结构域， Os11gRGA8 基因编码抗病蛋白属于 CC-NBSLRR抗病蛋白家族。Q-PCR结果显示，接种水稻干尖线虫 SAMN02420038的籼型常规稻‘大白谷’，与CK组‘大白谷’相比， Os11gRGA8基因表达量表现为上调，且 24 h内上调增幅较缓， 48 h时上调增幅突然上涨， 72 h时上调增幅又出现下降，表明该基因参与‘大白谷’天然免疫反应过程，在水稻干尖线虫侵染过程中起到一定抗性作用。

关键词: 猪圆环病毒II型, 猪圆环病毒II型, ORF4, 真核表达载体, ORF4抗体

Abstract: Os11gRGA8 gene was cloned and its bioinformatics were analyzed in order to explore the molecular mechanism of the indica rice (Oryza sativa L. ssp. Indica) and the role of resistance genes in the course of illness. Bioinformatics of Os11gRGA8 gene was analyzed, and its function in the process of Aphelenchoides besseyi infection was studied. Os11gRGA8 genes were in chromosome 11, containing 733 amino acids, its coding protein isoelectric point was 5.99 and the relative molecular mass was 84355.07. The sequence analysis showed that four structure domains RX- CC, AAA_22, NBS and LRR_4 were contained in its amino acid sequence and the disease-resistant protein encoded by Os11gRGA8 gene belonged to CC-NBS-LRR diseaseresistant protein family. Q- PCR results showed that, compared with CK rice seedlings group, the Indica inoculated by A. besseyi SAMN02420038 got a raised quantity of Os11gRGA8 gene expression and within 24 h the increase was relatively slow. Then the growth rose suddenly in 48 h, but the increase declined in 72 h,indicating that Os11gRGA8 gene was involved in Indica natural immune reaction process and played a role in the process of A. besseyi resistance infection.

陈俏丽,王峰,李丹蕾,孔畅仪,牛春阳,张瑞芝,王博文,刘爽,史欣悦,张荣. ‘大白谷’ CC-NBS-LRR蛋白编码基因抗干尖线虫侵染时空表达研究[J]. 中国农学通报, 2015, 31(21): 279-283.

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‘大白谷’ CC-NBS-LRR蛋白编码基因抗干尖线虫侵染时空表达研究

Spatial-temporal Expression of CC-NBS-LRR Protein-coding Gene Resisting to Infection of Aphelenchoides besseyi in Oryza sativa L. ssp. Indica

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