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中国农学通报

中国农学通报 ›› 2004, Vol. 20 ›› Issue (4): 12-12.

所属专题： 畜牧兽医

• 目次 • 上一篇    下一篇

不同稀释剂对猪冷冻精液的影响

江中良,李青旺,胡建宏,王立强,于永生,夏 凡   

  • 出版日期:2004-08-05 发布日期:2004-08-05

Study on the Technique of Boar Semen Freezing

JIANG Zhong-liang ,LI Qing-wang ,HU Jian-hong , WANG Li-qiang , YU Yong-sheng , XIA Fan   

  • Online:2004-08-05 Published:2004-08-05

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874

摘要： 手握法采集12月龄大约克种公猪精液，在37 ℃下离心，弃去精清，收集浓缩段富含精子部分，用7种不同的稀释液进行稀释，应用液氮熏蒸法制作颗粒冻精。结果表明，在7种稀释液中，7号稀释液 优于其他6种稀释液(p<0.05)；与其他的冷冻保护剂相比，甘油的冷冻保护性能较好(p<0.01)，其适宜浓度为2~4 ml·l-1；干解冻（40~45 ℃）效果优于湿解冻，解冻后精子活率达0.46~0.49（p<0.05）；稀释液中添加安钠咖可有效地延长精子的冻后存活时间(p<0.01)。

Abstract: The extenders and the cryoprotectant contents of frozen semen pellets were studied using the plate in boar. The results showed that the number 7 was the best extender (p<0.05)in seven groups, and the best cryoprotectant was glycerol (p<0.01).When the glycerol content was 2 percent (p<0.05),the sperm motility of frozen semen pellets was best in all treatment groups.The method of dry thawn of frozen semen pellets was better than that of wet thawn(p<0.05); the sperm motility was 0.46-0.49 after the frozen semen pellet was thawed at 40 to 45 ℃. supplyment in the extender could increase the thawn sperm survival time and motility effectively(p<0.01).