欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (11): 9-9.

• 目次 • 上一篇    下一篇

除精浆提高冷冻精液品质的实验研究

姬云涛,屈长青,曹斌云   

  • 出版日期:2005-11-05 发布日期:2005-11-05

Study on Improving Frozen-thawed Sperm Quality

Ji Yuntao, Qu Changqing, Cao Binyun   

  • Online:2005-11-05 Published:2005-11-05

摘要： 采用离心洗涤法去除精浆后，对精液进行冷冻处理。发现：1、除精浆精子冻后的活率、Ⅰ类顶体率和精子畸形率与未除精浆组存在显著差异(P<0.05)；2、除精浆使稀释后精液精浆中GOT和LDH活性明显升高(P<0.05)。从平衡后到解冻后各组GOT和LDH活性都呈上升趋势。但除精浆试验组比未除精浆试验组上升缓慢；3、除精浆试验组精子的穿卵率、卵内平均精子数都显著高于未除精浆试验组(P<0.05)；4除精浆离心洗涤液用生理盐水和脱脂乳-卵柠液，两者之间无显著差异(P>0.05)。

Abstract: The sperm being removed seminal plasma and not being removed seminal plasma by centrifugation were cryopreservated.1.The percentages of the frozen-thawed sperm of motility, normal acrosome and abnormal spermatozoa were significant difference between the tw