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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (12): 83-83.

所属专题： 生物技术； 园艺

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NAA和几种细胞分裂素对怀山药离体快繁的影响

唐 君,赵冬兰,张允刚   

  • 出版日期:2005-12-05 发布日期:2005-12-05

Influence of NAA and Some Kinds of Cytokinin on Dioscorea opposite Culture in vitro

Tang Jun, Zhao Donglan, Zhang Yungang   

  • Online:2005-12-05 Published:2005-12-05

摘要： 主要探讨了三种嘌呤型细胞分裂素：呋喃氨基嘌呤（KT）、苄氨基嘌呤（BAP）和异戊烯基腺嘌呤（2ip），对怀山药的两个品种：淮山药和凤山药离体培养的影响。结果表明：两品种在MS+KT2mg/L（单位下同）或2ip培养基上生长情况均较好，当附加NAA0.2时，一般可促进生根；但在培养基上，两品种差异明显，淮山药表现腋芽生长受抑制，而凤山药则易形成丛生芽，而当此培养基附加NAA0.2时，NAA与BAP可协同作用促进淮山药的腋芽生长，增加芽苗数，但对凤山药的生长则基本无影响。通过不同培养基对凤山药快繁效果的比较，筛选出MS+KT1+NAA0.2+0.1%活性碳为凤山药快繁较适宜的培养基。

Abstract: The paper discussed the effect of three kinds of cytokinin: kinetin(KT), benzylaminopurine(BAP) and isopentene group adenine(2ip) on Dioscorea opposite culture in virto. There are two varieties: Chinese yam rhizome and feng yam. Results were as follows: in MS basic medium+KT2mg/L or 2ip, the two varieties growed well. Appending NAA0.2 mg/L could promote radication commonly. But in MS+BAP2 mg/L there was obvious difference from the two varieties. Chinese yam rhizome axillary bud was restrainted. But feng yam was prone to form fascicular bud. When supplemented with NAA0.2 mg.L-1, NAA and BAP could accelerate Chinese yam rhizome axillary bud growth and increase the number of buds. But to feng yam made no difference. From comparison the effect of different media screened out MS+KT1+NAA0.2 mg/L +0.1%activated carbon were appropriate for feng yam.