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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (10): 390-395.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

福建乌龙茶种质离体保存研究Ⅱ 无菌系继代增殖与生根

郭玉琼   

  • 收稿日期:2008-07-30 修回日期:1900-01-01 出版日期:2008-10-05 发布日期:2008-10-05

In vitro Preservation of Germplasm of Oolong Tea Trees (Camellia sinensis) in FujianⅡ—Subculturing Multiplication and Rooting Formation of Aseptic Lines from Adult Stem Nodes

  • Received:2008-07-30 Revised:1900-01-01 Online:2008-10-05 Published:2008-10-05

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摘要： 本试验以铁观音茶树成年茎段离体培养诱导的丛生芽作为研究材料，探讨无菌系的继代增殖和生根。试验结果表明：适宜茶树成年茎段无菌系继代增殖的培养基为1/2MS＋1.0mg/L6-BA＋0.2mg/LIAA＋0.2mg/LGA3，培养基中附加8g/L琼脂糖，30g/L蔗糖，pH5.6。在此继代培养基上进行增殖，茶树成年茎段平均增殖率为7.57，最高增殖率可达13。适合茶树成年茎段离体培养生根的培养基是1/2MS＋3mg/LIBA＋30g/L蔗糖＋7g/L琼脂糖；研究还表明随着继代生长时间的延长，生根率提高，最早生根的天数缩短。

Abstract: In this experiment the ramification from tea adult stem nodes was used as materials for the preliminary study on subculturing multiplication and rooting formation in Camellia sinensis cv Tieguanyin. The obtained results showed that the best medium for subculturing multiplication of tea adult stem nodes was 1/2MS＋1.0 mg/L6-BA＋0.2mg/LIAA＋0.2mg/LGA3 supplemented with 8g/L agarose and 30g/L sucrose, pH5.6. The average multiplication rate was 7.57 and the highest could come up to 13. The optimal medium for rooting formation of aseptic lines from adult stem nodes was 1/2MS＋3mg/LIBA＋30g/L sucrose＋7g/L agarose; further more the rooting rate improved with prolonging subculturing time, while the days of rooting formation shortened.