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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (2): 16-19.

• 生物技术科学 • 上一篇    下一篇

植物生长调节剂对3个柑橘品种形态建成的影响

王彩霞   

  • 收稿日期:2008-10-10 修回日期:2008-10-13 出版日期:2009-01-20 发布日期:2009-01-20

Effect of Plant Growth Regulators on the Morphogenesis of Three Citrus Species

WANG Cai-Xia   

  • Received:2008-10-10 Revised:2008-10-13 Online:2009-01-20 Published:2009-01-20

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摘要： 摘 要：本研究以墨西哥来檬、甜橙和香橼为材料，采用茎段培养方法，研究了植物生长调节剂不同配比对3个柑橘品种腋芽萌发、增殖和生根的影响，结果表明，以MT为基本培养基，3个柑橘品种在附加BAP 2.0 mg/L和IBA 0.1 mg/L的培养基中腋芽萌发效果最好，离体植株在附加BAP 1.0 mg/L和IBA 0.2 mg/L的培养基中增殖系数最大。此外，以1/2MT为基本培养基，附加NAA 2.0 mg/L 和IBA 0.25 mg/L时，适宜于墨西哥来檬和甜橙的生根，而单独使用NAA 3.0 mg/L则易于香橼的生根。离体小植株的移栽成活率均在90%以上。

关键词: 河北坝上地区, 河北坝上地区, 下垫面, 风蚀沉积量, 粗糙度, 植被盖度

Abstract: Abstract: The effects of plant growth regulators on the regeneration of buds and shoots, multiplication and root formation of in vitro cultures from three citrus species were identified by stem culture. Results showed that MT medium supplemented with BAP 2.0 mg/L and IBA 0.1 mg/L was suitable for the regeneration of buds and shoots for three citrus species. The highest shoots multiplication index of three citrus species was obtained on a medium containing BAP 1.0 mg/L and IBA 0.2 mg/L. In addition, 1/2 MT medium supplemented with NAA 3.0 mg/L alone was best for the rooting of Etrog citron (C. medica L.), whereas the most number and the highest length of roots were produced on a treatment containing both NAA 2.0 mg/L and IBA 0.25 mg/L for Mexican lime (Citrus aurantifolia (Christm.) Swingle) and sweet orange (C. sinensis (L.) Osbeck). Plantlets survived of three citrus species were all more than 90% in soil under greenhouse conditions.