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中国农学通报 ›› 2011, Vol. 27 ›› Issue (6): 155-160.

所属专题: 园艺

• 林学 园艺 园林 • 上一篇    下一篇

蒙古扁桃AMF多样性及其AMF接种效应研究

王琚钢 白淑兰 盖京苹 慈忠玲 方 亮   

  • 收稿日期:2010-10-19 修回日期:2010-11-24 出版日期:2011-03-20 发布日期:2011-03-20
  • 基金资助:

    国家自然基金

Study on Arbuscular Mycorrhiza Fungi Diversity and Inoculation Effect of Prunus mongolica

  • Received:2010-10-19 Revised:2010-11-24 Online:2011-03-20 Published:2011-03-20

摘要:

以内蒙古西部沙漠地区蒙古扁桃(Prunus mongolica)为对象,对野外采集的蒙古扁桃营养根进行外生菌根形态观察及采用Phillips﹠Hayman染色方法观察根内丛枝菌根结构,同时通过形态学方法对根际孢子进行鉴定。结果表明:所采样品未发现外生菌根侵染,而丛枝菌根的侵染频度高达97%以上,在根际土中共鉴定出丛枝菌根真菌4属11种,和一个未知种,其中瑞氏无梗囊霉和摩西球囊霉是蒙古扁桃根际土中的优势种。另外,在实验室条件下用摩西球囊霉和地表球囊霉对蒙古扁桃进行人工接种试验,结果表明:摩西球囊霉接种处理下,蒙古扁桃营养根细胞内形成菌丝、丛枝、泡囊等典型的丛枝菌根结构,而地表球囊霉接种处理在蒙古扁桃营养根内未形成丛枝菌根结构;摩西球囊霉在形成丛枝菌根后明显促进了蒙古扁桃的生长。

关键词: 物质转化, 物质转化

Abstract:

Mycorrhizal status of Prunus mongolica of western Inner Mongolia in the desert region was studied by observing the ectomycorrhiza morphological and the arbuscular mycrrhizal structure of the nutritive root. Ectomycorrhiza colonization was not found in the present study, while arbuscular mycrrhiza colonized quite well, and the highest frequency of colonization was 97%. Eleven arbuscular mycorrhizal fungi species of four genera and one uncertain species were identified by the morphologiacal identification of the spores, among which Acaulosopra rehmii and Glomus mosseae were the dominant species. In addition, Glomus mosseae and Glomous versiforme were inoculated on Prunus mongolica, and the result showed that the nutritive root of Prunus mongolica and the Glomus mosseae can form the typical arbuscular mycrrhizal structure as hyphae, vesicles and arbuscular, but not with the Glomous versiforme. Glomus mosseae promoted the growth of Prunus mongolica.

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