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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (26): 51-54.

所属专题： 生物技术； 畜牧兽医； 园艺

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

黄芪多糖(APS)拮抗铅镉联合致鸡脾脏细胞凋亡作用的研究

费东亮 苏禹刚 肖银霞 白刃 王令 吴思思   

  • 收稿日期:2012-03-26 修回日期:2012-05-19 出版日期:2012-09-15 发布日期:2012-09-15

Rivalry Effect of Astragalus Polysaccharides (APS) Against Splenocytes Apoptosis by Lead Cadmium Take Care, Toxicosis in Chicken

  • Received:2012-03-26 Revised:2012-05-19 Online:2012-09-15 Published:2012-09-15

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摘要：

为了探讨黄芪多糖注射液对铅镉联合致鸡脾脏细胞凋亡的拮抗作用。采用TUNEL法、RT-PCR和DNA ladder方法检测分别对对照组、铅镉组和黄芪多糖-铅镉组的鸡脾细胞凋亡率和脾淋巴细胞凋亡基因Bcl-2 mRNA转录水平。结果表明：与对照组比较,试验组的细胞凋亡率明显升高，Bcl-2mRNA表达水平降低；试验组和对照组差异显著或差异极显著，并且黄芪多糖-铅镉组数据介于对照组和铅镉组之间。试验结果显示：黄芪多糖注射液对铅镉联合致鸡脏脾细胞凋亡具有明显的拮抗作用。

关键词: 证明商标, 证明商标

Abstract:

The purpose of this study was to explore APS against Splenocytes apoptosis by lead cadmium toxicosis in chicken. Apoptosis rate of splenocytes and transcriptional level of Bcl-2 mRNA were detected by TUNEL method and RT-PCR in control, lead cadmium and APS-lead cadmium group. The results showed that: there were higher apoptotic rate and lower transcriptional level of Bcl-2 mRNA, compared with the controls; There were significant or very significant difference in control and experimental groups, and the data of APS-lead cadmium group was between control and lead cadmium groups. It suggested that APS could resisted splenocytes apoptosis by lead cadmium toxicosis significantly.