欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (10): 203-207.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

甘蓝枯萎病菌原生质体的制备与再生条件的优化

李伶俐 严红 李兴红 姜于兰 向红琼 杨腊英 燕继晔   

  • 收稿日期:2010-11-11 修回日期:2010-12-14 出版日期:2011-05-15 发布日期:2011-05-15
  • 基金资助:

    农业部公益性行业（农业）科研专项经费“由尖孢镰刀菌引起的作物土传病害综合防控技术研究”

Optimizing Method for Protoplast Preparation and Regeneration in Fusarium oxysporum f.sp. conglutinans

  • Received:2010-11-11 Revised:2010-12-14 Online:2011-05-15 Published:2011-05-15

PDF (PC)

1036

摘要：

建立甘蓝枯萎病菌（Fusarium oxysporum f.sp. conglutinans）高效遗传转化体系的途径之一是获得其具有再生功能的原生质体。本研究通过对甘蓝枯萎病菌菌丝摇培时间、菌丝的酶解时间、保护原生质体的渗透压稳定剂的种类和浓度等因素进行优化，建立了制备甘蓝枯萎病菌原生质体的高效、稳定体系，并在SR固体培养基上实现了甘蓝枯萎病菌原生质体的再生，再生率可达21.13%。该体系的获得将为下一步甘蓝枯萎病菌高效遗传转化体系的建立和致病机理的解析奠定基础。

关键词: 真核表达, 真核表达

Abstract:

Protoplast preparation and regeneration is one of the most important methods for constructing genetic transformation in Fusarium oxysporum f.sp. conglutinans. The culture time of mycelium, the enzymolysis time, the osmotic stabilizer typer and the osmotic stabilizer concentration were optimized, at last the author gained an efficient method for protoplast preparation and regeneration of F. oxysporum. Furthermore, the protoplast can regenerate on regeneration medium, the regeneration rate of the protoplast was 21.13%. The system provided an basic for F. oxysporum genetic transformation and pathogenicity analysis in molecular levels.

中图分类号: