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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (6): 193-196.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

植物叶片提取物对枯草芽孢杆菌抑制石榴枯萎病菌的反应

汤东生 但朝辉 毛忠顺 何霞红   

  • 收稿日期:2010-11-19 修回日期:2010-12-05 出版日期:2011-03-20 发布日期:2011-03-20
  • 基金资助:

    作物多样性对病虫害的生态调控和对土壤地力的影响

The Influence of Plant Leaves Extracts on the Inhibition of Bacillus subtilis to Pomegranate Wilt Pathogen (Ceratocystis fimbriata)

  • Received:2010-11-19 Revised:2010-12-05 Online:2011-03-20 Published:2011-03-20

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摘要：

为评价利用生防细菌枯草芽孢杆菌（Bacillus subtilis）和果树多样性间栽控制石榴枯萎病（Ceratocystis fimbriata）的作用效果，本研究测定了生防菌株枯草芽孢杆菌BS9601-Y1、Y2对石榴枯萎病菌的抑制作用、石榴园间作树木小枣、枇杷、桉树和石榴叶片乙醇粗提物对石榴枯萎病菌和生防菌生长的影响。结果表明：枯草芽孢杆菌对石榴枯萎病菌的拮抗作用为：BS9601-Y2高于BS9601-Y1，但当菌株培养48 h后，对石榴枯萎病菌的抑制率均达到100%。在供试叶片材料中，1 mg/mL的小枣和枇杷叶片乙醇粗提取物对石榴枯萎病菌的抑制效果较高，分别为29.1%、44.6%；4种叶片乙醇粗取物对生防菌的抑制作用较强，在0.5 mg/mL时，抑制率都达到了98%。由此推测，石榴园间作树木叶片的提取物影响了枯草芽孢杆菌的增殖；在石榴园不能同时使用间栽果树和施用枯草芽孢杆菌剂的方式来控制石榴枯萎病。

关键词: 药效试验, 药效试验

Abstract:

The present study was carried out to evaluate possibility of application combination of Bacillus subtilus with intercropping fruit trees in pomegranate plantation to control pomegranate wilt pathogen Ceratocystis fimbriata. The role of strains of B. subtilis BS9601-Y1 and Y2 on the inhibition of C. fimbriata and leaves raw ethanol extracts of Eriobotrya japonica, Punica granatum, Zizyphus jujube, Eucalyptus spp were examined in laboratory condition. The results showed that the two strains of B. subtilis had strong inhibitory effect on C. fimbriata. The effect of Y2 was higher than Y1. The role of leaves of Z. jujube and E. japonica in inhibiting of C. fimbriata growth was stronger than other plants. The inhibition ratio of Z. jujube and E japonica were 29.1% and 44.6%, respectively. All leaves raw ethanol extracts showed strong inhibitory effect on B. subtilis. 0.5 mg/mL leaves extracts could inhibit 98% B. subtilis growth. In a word, the leaves extracts of trees planted in pomegranate yard influenced colonization and multiplication of B. subtilis. Therefore, intercropping fruit trees in pomegranate yard prevented using B. subtilis to control pomegranate wilt.