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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (10): 239-242.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

BVDV基因E0在人参发根的转化及其分子检测

李然 臧 埔 郜玉钢 马 琳 王亚星 李 学 李 萍 张连学   

  • 收稿日期:2010-11-24 修回日期:2010-12-29 出版日期:2011-05-15 发布日期:2011-05-15
  • 基金资助:

    梅花鹿高效养殖加工关键技术的研究与应用;国家科技支撑计划项目;中国博士后科学基金项目

Transformation and Molecular Detection of BVDV Gene E0 in Ginseng Hairy Roots

  • Received:2010-11-24 Revised:2010-12-29 Online:2011-05-15 Published:2011-05-15

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摘要：

将重组鹿源BVDV基因E0植物表达载体（pBI121/E0），通过发根农杆菌Ri介导，用叶盘法转化得到人参发根，分别用PCR、RT-PCR检测其转化情况。结果表明：获得了转鹿源BVDV基因E0人参发根，鹿源BVDV基因E0已整合到人参发根基因组中并得到了转录，为研制转基因人参发根疫苗提供了科学依据和材料。

关键词: 抗性评价, 抗性评价

Abstract:

Ginseng hairy roots were induced by leaf disc method, and recombinant plant expression vector (pBI121/E0) of BVDV gene E0 of deer was transformed into ginseng hairy roots by Agobacterium rhizgenses with Ri-plasmid. E0 in ginseng hairy roots was detected by PCR, RT-PCR methods. The results indicated that E0 transgenic ginseng hairy roots were obtained. E0 was transferred into Ginseng hairy roots, and expressed in ginseng hairy roots.

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