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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (13): 157-160.

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

粗根鸢尾染色体制片技术及核型分析

毕晓颖 赵芷唯 郑洋 李东升   

  • 收稿日期:2010-12-09 修回日期:2011-01-31 出版日期:2011-06-05 发布日期:2011-06-05
  • 基金资助:

    辽宁省教育厅科学技术研究项目

Staining and Slide-preparing Technique of Chromosome and Karyotype Analysis of Iris tigridia

  • Received:2010-12-09 Revised:2011-01-31 Online:2011-06-05 Published:2011-06-05

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摘要：

以粗根鸢尾根尖为试材，研究取样时间、预处理液、解离时间等对其染色体制片的影响，并以此最佳条件进行粗根鸢尾核型分析，为其细胞学研究及开展杂交育种提供参考。结果表明，根尖取样以上午8:00～11:00从田间植株取材为佳；用2 mmol/L 8-羟基喹啉溶液预处理2.5 h，经卡诺固定液固定2 h后，在5 mol/L盐酸常温下解离10 min，卡宝品红溶液染色压片镜检，能取得良好的制片效果；粗根鸢尾的染色体数目为2n=18，核型公式为2n=2x=18=2M+10m+6sm，核型属于2A型。

关键词: 营养状况, 营养状况

Abstract:

The root tips of Iris tigridia were used to study the effects of sampling time, pre-treating solution and dissociation time on slide-preparing for chromosome and karyotype analysis. The aim was to provide the references for its cytology research and crossbreeding. The results showed that the optimal sampling time of root tips was 8:00-11:00 am in the field. The best process of staining and slide-preparing technique was that the root tips were pretreated 2.5 h with 2 mmol/L 8-hydroxy quinoline solution, and then it was fixed with Carnoy solution for 2 h, associated with 5 mol/L HCl for 10 min at room temperature and stained with Carbol fuchsin solution. The chromosome number of Iris tigridia was 2n=18. Karyotype formula was 2n=2x=18=2M+10m+6sm. The karyotype classification belonged to 2A type.