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中国农学通报 ›› 2005, Vol. 21 ›› Issue (3): 70-70.

所属专题: 园艺

• 目次 • 上一篇    下一篇

低能Ar+注入樱桃萝卜点点红种子后的生物学效应

梁秋霞,曹刚强,黄群策,秦广雍,马青荣   

  • 出版日期:2005-03-05 发布日期:2005-03-05

Study on Biological Effects of Low Energy Ar+ Ions Implantation in Dian Dian Hong

Liang Qiuxia, Cao Gangqian, Huang Qunce, Qin Guangyong , Ma Qingrong   

  • Online:2005-03-05 Published:2005-03-05

摘要: 选取樱桃萝卜点点红为材料,对低能Ar+离子注入其干种子后的生物学效应进行了研究。考察了其发芽率、成活率与低能Ar+注入剂量的关系,对低能Ar+注入点点红种子后其过氧化物酶(POD)的活性进行了测定,并对POD酶谱及酯酶(Est)同工酶酶谱进行分析。研究结果表明,不同剂量的低能Ar+注入都会引起种子发芽率、成活率及不同阶段的生长和发育产生变异;在1×1017Ar+/cm2与3×1017Ar+/cm2剂量之间点点红种子的发芽率随注入剂量的增加呈现出“马鞍型”曲线的变化趋势;成活率的变化规律与发芽率的变化规律基本一致;低剂量注入时,其POD活性随剂量的增加而升高;高剂量注入时,其POD活性随剂量的增加而降低。同工酶图谱中,POD在2.5×1017Ar+/cm2和3.0×1017Ar+/cm2增加一条酶带,且1.5×1017Ar+/cm2和3.0×1017Ar+/cm2高剂量注入时有缺失现象;酯酶(Est)在1.5×1017Ar+/cm2和3.0×1017Ar+/cm2剂量注入时酶带数增加,且变深。

Abstract: In this study,We choose Dian dian hong as materials to study the biological effect of low energy Ar+ Ions implantation。The relationships between the materials of the budding rate and the survival rate and the irradiated dosages of low energy Ar+ Ions implantation are explored. Further more, we measure the activity of peroxidase isozyme and the kind of peroxidase isozymes and esterase isozyme in leaves of dian dian hong after implantation by low energy Ar+ Ions. The results improve that different mutation dosages of Ar+ Ions implantation can all make dissociation in the materials of budding rate and the survival rate and their growth and development in each step. Between the dosage of 1×1017Ar+/cm2 and 3×1017Ar+/cm2 the budding rate curve of Dian Dian Hong could take a “saddle pattern” with the increased Ar+ Ions implanting dosage. The survival rate takes the similar change regulation with the budding rate, but has lower amplitude. With Ar+ Ions impanted in low dose, the activity of peroxidase isozymes increase along the Ar+ Ions implanting dosage, but when Ar+ Ions are implanted in higher dose, the peroxidase activity decrease. The band of isozymes suggest that POD increase one band in the dosage of 2.5×1017Ar+/cm2 and 3×1017Ar+/cm2, but these lose in the dosage of 1.5×1017Ar+/cm2 and 3×1017Ar+/cm2 ; the amount of Est bands increase in the dosage of 1.5×1017Ar+/cm2 and 3×1017Ar+/cm2.