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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (3): 81-81.

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香石竹茎尖试管苗继代培养玻璃化现象的研究

黄宇翔,李章汀,张晓耕   

  • 出版日期:2005-03-05 发布日期:2005-03-05

Study on Decrease of Vitrification in Subculture of Carnation

Huang Yuxiang, Li Zhangting, Zhang Xiaogeng   

  • Online:2005-03-05 Published:2005-03-05

摘要： 香石竹红色品种茎尖试管苗继代培养玻璃化现象是香石竹脱毒试管苗生产的一个主要障碍。采用改善光照，在培养中提高糖和琼脂的浓度，降低细胞分裂素的用量，对克服香石竹茎尖试管苗继代培养玻璃化有明显效果，但对一些红色系品种效果并不理想，继代培养玻璃苗率仍达61%以上。

Abstract: Vitrification is the main obstacle in subculture of carnation plantlets in vitro, which were induced from shoot tips. The effective way to decrease vitrification is to increase the concentration of sucrose and agar in the medium, to improve luminosity, and to decrease the concentration of cell devision hormone. However, this way has little effect on some of red-colored species, which have a high vitrification frequency of over 61% in subculture.