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中国农学通报 ›› 2011, Vol. 27 ›› Issue (22): 187-191.

所属专题: 食用菌 食用菌

• 林学 园艺 园林 • 上一篇    下一篇

真姬菇与凤尾菇融合菌株筛选鉴定及营养成分分析

李晓静 黄丽萍 刘建忠 孙淑静 胡开辉   

  • 收稿日期:2010-12-27 修回日期:2011-01-05 出版日期:2011-09-05 发布日期:2011-09-05
  • 基金资助:

    食用菌木质素降解酶遗传转化体系建立及其在育种中的应用研究;真姬菇品种生产性能改良及其相关性研究;食用菌育种漆酶转化体系的建立及机理分析;漆酶与食用菌生产周期关系研究及其应用

Identification and Nutritional Components Analysis of Hypsizigus marmoreus and Pleurotus sajor-caju Fusion Strain

  • Received:2010-12-27 Revised:2011-01-05 Online:2011-09-05 Published:2011-09-05

摘要:

本研究以漆酶为筛选目标,根据出发菌株凤尾菇与白玉菇漆酶活性的差异,采用RB-PDA平板显色技术与分子标记相结合的方法快速、高效地筛选出具有漆酶活性的凤尾菇与白玉菇融合菌株IV19C,采用常规方法对白玉菇与凤尾菇融合菌株IV19C进行了营养成分分析及安全性评价。结果表明,融合菌株IV19C与两个出发菌株不同,水分含量(90.0%)与两出发菌株无明显差异,IV19C灰分含量(9.81%)、蛋白质含量(36.0%),明显高于凤尾菇;粗纤维含量(18.3%)、粗脂肪含量(2.50%)高于白玉菇,含有七种必需氨基酸,谷氨酸和天冬氨酸含量丰富,但缺乏色氨酸;该样品急性毒性试验结果为MTD>15 g/kg体重,根据急性毒性剂量分级属无毒。以上实验结果说明融合菌株IV19C营养丰富,食用安全,有良好的开发应用前景。

关键词: 最高气温 最低气温 非对称变化, 最高气温 最低气温 非对称变化

Abstract:

Fusion strain IV19C of Hypsizigus marmoreus and Pleurotus sajor-caju which had laccase activity was selected quickly by RB-PDA plate and molecular marker method on the basis of the difference of Hypsizigus marmoreus and Pleurotus sajor-caju laccase activity. The nutrition components and safety of fusion strain IV19C were analyzed and evaluated with GB (national standards methods) and other related methods. The results showed that fusion strain IV19C was different from Hypsizigus marmoreus and Pleurotus sajor-caju, and the water content of strain IV19C was 90.0% and there was no significant difference with Hypsizigus marmoreus and Pleurotus sajor-caju. However, the ash and crude protein content of strain IV19C were 9.81% and 36.0%, respectively. They were significantly higher than that of Pleurotus sajor-caju. The crude fiber and crude fat contents were 18.3% and 2.50%, respectively. They were higher than that of Hypsizigus marmoreus. Seven essential amino acids were contained. Glutamic acid and aspartic acid were rich but tryptophan was deficient. The fruit body of strain IV19C was safe to eat based on acute toxicity classification. IV19C was rich in nutrients, safe to eat, and had a good prospect.

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