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中国农学通报 ›› 2011, Vol. 27 ›› Issue (29): 23-26.

所属专题: 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

陕北绒山羊冷冻精液稀释液添加中草药试验

赵文俊 李新春 毕台飞   

  • 收稿日期:2011-03-29 修回日期:2011-04-16 出版日期:2011-11-15 发布日期:2011-11-15
  • 基金资助:

    陕西省教育厅专项科研计划;榆林市科技项目

Test on Frozen Dilute Sperm of Extracts Adding Medicinal Herb in Northern Shaanxi Cashmere Goat

  • Received:2011-03-29 Revised:2011-04-16 Online:2011-11-15 Published:2011-11-15

摘要:

本试验旨在探究陕北绒山羊冷冻精液稀释液添加以淫羊藿为主的中草药提取液,对解冻后精子质量的影响。试验分取四种单方和复方提取液按 5%,10%,15%和20%四种体积分数添加到稀释液中,制作细管冻精,比较解冻后精子活率,畸形率,顶体异常率,常温下存活时间,有效存活时间与对照组的差异。结果显示:陕北绒山羊冷冻精液稀释液添加中草药提取液,可以提高冷冻精液解冻后精液的质量。其中以添加淫羊藿、巴戟天、菟丝子、甘草等中草药的复方配方在占冷冻稀释液总量的10%时,解冻后精液质量最好:其平均值为活率0.433,精子畸形率14.28%,顶体异常率16.64%,精子存活时间和有效存活时间分别为8.00h、4.56h。

关键词: 遥感监测, 遥感监测

Abstract:

The present study was designed to investigate the effect of extracts of medicinal herb which major component is barrenwort on frozen dilute sperm quality in northern shaanxi cashmere goat. Four simple prescriptions and one compound prescription were added into dilute sperm, according to volume fraction 5%, 10%, 15% and 20%, respectively, and then frozen semen straws. The indexes of sperm activity rate, deformity rate, acrosome abnormity rate, survival time at normal temperature, effective survival time were analyzed, compared to that of control group. Results indicated that thaw sperm quality was the best among these groups when added 10% compound prescription extracts of barrenwort, morinda officinalis, dodder, actinolite, glycyrrhiza. Average of sperm activity rate, deformity rate, acrosome abnormity rate, survival time at normal temperature, effective survival time were 0.433, 14.28%, 16.64%, 8.00h and 4.56h respectively when added compound prescription into spermatozoa diluent. Results support that extracts of medicinal herb improve thaw sperm quality.