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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (22): 214-218.

• 林学 园艺 园林 • 上一篇    下一篇

莲雾SRAP反应体系的优化及其应用

蔡元保 杨祥燕 黄明忠 曾黎明 崔明勇 陈显国 林玉虹   

  • 收稿日期:2011-04-25 修回日期:2011-05-19 出版日期:2011-09-05 发布日期:2011-09-05
  • 基金资助:

    广西壮族自治区直属公益性科研院所基本科研业务费专项资金项目

Optimization of SRAP-PCR System and its Application in Wax Apple (Syzygium samarangens)

  • Received:2011-04-25 Revised:2011-05-19 Online:2011-09-05 Published:2011-09-05

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摘要：

以莲雾DNA为模板，应用正交设计法对SRAP反应体系中的各个主要影响因素进行了优化筛选。结果表明，20 μL反应体系中各组分的最适浓度或用量分别为：1×Buffer，3.0 mmol/L Mg2+，0.3 mmol/L dNTPs，0.4 μmol/L引物，0.5 U Taq DNA聚合酶和40 ng模板DNA。利用该优化体系通过64对SRAP引物组合对7份连雾进行了SRAP-PCR扩增，证实了该体系具有稳定可靠、重复性好、多态性较强等特点。

关键词: 木材, 木材, 阻燃剂, 增重率, 失重率, 燃烧性, 浸渍处理

Abstract:

The main influential factors of SRAP reaction system were optimized for genomic DNA in wax apple (Syzygium samarangens) by orthogonal design. The results showed that the optimum concentration was 1×Buffer, Mg2+ 3.0 mmol/L, dNTPs 0.3 mmol/L, primer 0.4 μmol/L, Taq DNA polymerase 0.5 U, 40 ng template DNA with a total volume of 20 μL reaction solution. The molecular identification of 7 varieties of wax apple were achieved by 64 SRAP primer combinations with this optimum system which could provide clear, reliable and repeatable abundant polymorphisms molecular markers.