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中国农学通报 ›› 2005, Vol. 21 ›› Issue (4): 59-59.

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Phenotype Variations in Plant Regeneration of Upland Cotton (Gossypium hirsutum L.)

迟吉娜,马峙英,韩改英,李喜焕,王彦霞,王省芬   

  • 出版日期:2005-04-05 发布日期:2005-04-05

Phenotype Variations in Plant Regeneration of Upland Cotton (Gossypium hirsutum L.)

Chi Jina, Ma Zhiying, Han Gaiying, Li Xihuan, Wang Yanxia, Wang Xingfen   

  • Online:2005-04-05 Published:2005-04-05

摘要: 以陆地棉冀无2031无菌苗下胚轴为外植体,在MS中附加激素0.1mg/L IAA+0.1mg/L 2,4-D+0.1mg/L KT的培养基上诱导出愈伤组织。选择疏松愈伤组织继代到附加0.1mg/L ZT的培养基中,经2~3个月的继代培养诱导出颗粒状疏松胚性愈伤组织。胚性愈伤组织在无激素、每升加2g谷氨酰胺和0.5g天门冬酰胺的胚性愈伤增殖萌发培养基上培养2个月左右,可陆续形成不同时期的体胚苗。对冀无2031不同时期的体细胞萌发后的体胚苗进行观察,发现了真叶畸形苗、子叶节生根胚、胚轴侧生根胚、腋芽丛生苗和以腋

Abstract: A variety of upland cotton, Jiwu2031, was studied on somatic embryogenesis and plant regeneration. Callus was initiated from hypocotyls explants on a callus initiation medium, modified MS with 0.1mg/L IAA+0.1mg/L2,4-D+0.1mg/L KT. Friable callus was period