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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (5): 1-1.

所属专题： 生物技术； 畜牧兽医

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山羊骨髓间充质干细胞生物学性能鉴定 及诱导分化为心肌细胞

庞全海,,张慧茹,华进联,马勇江,窦忠英   

  • 出版日期:2005-05-05 发布日期:2005-05-05

Identification and Differentiation into Cardiomyocytes of Goat Bone Marrow Mesenchymal Stem Cells in vitro

Pang Quanhai,, Zhang Huiru, Hua Jinlian, Ma Yongjiang, Dou Zhongying   

  • Online:2005-05-05 Published:2005-05-05

摘要： 研究了关中奶山羊骨髓间充质干细胞的分离、培养和生物学特性，并在体外诱导分化为心肌细胞表型。结果表明，山羊MSCs具有很好增殖能力；能够耐受反复冷冻和解冻；表达细胞表面标志CD44、CD105、CD166，弱表达CD34、CD106，而不表达CD14、CD45。该类细胞用5-氮胞苷诱导分化后，能够表达心肌α-actin，并呈PAS染色阳性。

Abstract: The Guanzhong dairy goats were used in our experiment to study the characteristics of their bone marrow mesenchymal stem cells (BM-MSCs), such as isolation, culture, and identification. The results indicate that there are excellent potentials for the goat mesenchymal-like stem cells derived from goat bone marrow to proliferate and to resist freezing and thawing repeatedly. The goat mesenchymal-like stem cells can express cellular surface markers CD34, CD44, CD105, CD106, CD 166, and TGF-α, but not CD14 and CD45. And after treated by 5-azacytidine in vitro, they express cellular surface marker of cardiac α-actin and Schiff’s stain positive.