Abstract: Pepper pot experiments were conducted together with the application of different microorganism and residue of mushroom medium in a glass greenhouse to study the influences of microbial manures (microorganisms + residue of mushroom medium) on pepper yield，nutrient uptake and soil nutrients by measuring yield of pepper, and by determining changes of soil alkaline hydrolysis N, available P ，available K contents and total N, P, K contents of pepper plant after harvesting. The results indicated that microbial manures increased pepper yield and were conducive to improvement of soil fertility and total N, P, K contents of pepper plant. Treatment 3（M+ residue of mushroom medium）had the best effects among all the microbial treatments.