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中国农学通报 ›› 2012, Vol. 28 ›› Issue (12): 112-117.doi: 10.11924/j.issn.1000-6850.2011-3121

• 农学 农业基础科学 • 上一篇    下一篇

高效降解棉酚菌株的筛选鉴定及毒性试验

张丛 李佳 袁洪水 郝志敏 朱宝成   

  • 收稿日期:2011-10-25 修回日期:2011-12-23 出版日期:2012-04-25 发布日期:2012-04-25

Isolation and Identification of Strains for Digesting Free-gossypol and Toxicity Test

  • Received:2011-10-25 Revised:2011-12-23 Online:2012-04-25 Published:2012-04-25

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摘要：

为了筛选一株能高效降解棉籽饼中棉酚毒素的菌株，以棉酚为唯一碳源，从棉田土壤中分离筛选对棉酚具有降解作用的菌株，得到具有较高降解活性的菌株M-9，其降解率达到94.74%，对其进行了菌株形态观察和生理生化鉴定并结合其16S rDNA序列，初步确定其为枯草芽孢杆菌(Bacillus subtilis)。通过对M-9菌株的脱毒效果研究表明，其具有降解棉籽饼中游离棉酚的能力，脱毒后棉籽饼中游离棉酚的含量降至33.18 mg/kg，符合中国国家标准饲料卫生标准规定(GB13078—2001)：饲料原料棉籽饼中游离棉酚含量≤1200 mg/kg。并进行毒性饲喂试验，从而确定菌株M-9处理后的棉籽饼粉对小白鼠无毒性。

关键词: 田园城市, 田园城市

Abstract:

To obtain high efficient gossypol-degraded bacteria, a strain named M-9 was isolated from a soil microcosm by used the gossypol as the only carbon resource and investigated due to the finding that it could degrade free-gossypol. After observe its morphology, analyzed its biochemical characteristic and combine with 16S rDNA sequence, it was identified as the Bacillus subtilis preliminarily. The detoxification research showed that, the M-9 bacterial strain had the ability to be degraded the free gossypol in the cottonseed cake, and the rate of degradation was 94.74%, residual quantity was up to our national feed hygiene standard (GB13078-2001), the free gossypol content of the feedstuff cottonseed cake was no more than 1200 mg/kg. And via toxicity feeding test, we make sure that, the treatment to the bacterial strain M-9 had reduced the toxicity of gossypol.