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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (26): 247-251.

所属专题： 植物保护

• 资源 环境 生态 土壤 气象 • 上一篇    下一篇

草酸青霉ZHJ6固定化后对甲胺磷农药降解的影响

赵仁邦 靳存华 刘卫华   

  • 收稿日期:2011-11-08 修回日期:2012-01-21 出版日期:2012-09-15 发布日期:2012-09-15

The Effects of Immobilization of Penicillium oxalicum ZHJ6 Strain on the Biodegradation of Methamidophos

  • Received:2011-11-08 Revised:2012-01-21 Online:2012-09-15 Published:2012-09-15

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摘要：

对能够降解有机磷农药的草酸青霉ZHJ6进行固定化研究，了解固定化后菌丝是否提高降解有机磷农药的能力和对高浓度农药的耐受能力。用包埋法和载体结合法固定化菌丝，并对固定化以后菌丝降解甲胺磷的影响因素进行试验。结果表明聚酯无纺布结合法固定化菌丝对甲胺磷的降解率要高于游离菌丝，在同样的温度、pH或者初始甲胺磷浓度条件下甲胺磷的降解率都得到了提高，最佳条件为pH为5.0，温度为25℃。而且固定化后对氧化乐果、草甘膦、辛硫磷以及用河水和土壤模拟的甲胺磷农药的降解能力都比游离菌丝的高。

关键词: 主基因-多基因遗传, 主基因-多基因遗传

Abstract:

The immobilization of Penicillium oxalicum ZHJ6 strain which could degrade organ phosphorous pesticide was studied in order to investigate whether the immobilized Penicillium oxalicum ZHJ6 strain could keep the activity of degradation of organophosphorus pesticide and suffer from high concentration of organophosphorus pesticide. The investment and combined methods were used to immobilize the strain, and the influence factors of degradation ratio of methamidophos were tested. The result showed that the degradation ration of methamidophos by immobilzated ZHJ6 strain in polyester nonwoven fabric was higher than by free ZHJ6 strain. The degradation ratio was raised by immobilzated ZHJ6 strain in polyester nonwoven fabric in the same temperature, pH or the concentration of methamidophos. The optimal condition for degradation of methamidophos was pH 5.0 and 25℃. And the degradation ratios of folimat, phoxim, glyphosate and methamidophos by the immobilization strain in simulation wastewater through soil or river water were also higher than that by free ZHJ6 strain.