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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (24): 250-255.

所属专题： 生物技术

• 植物保护 农药 • 上一篇    下一篇

裂褶菌蛋白粗提液抗植物病毒活性初探

李洋 周莹 李兴红 王红清   

  • 收稿日期:2011-12-06 修回日期:2012-03-30 出版日期:2012-08-25 发布日期:2012-08-25

The first study on the activity of anti-tobacco mosaic virus and anti- Pepper virus of crude protein from Schizophyllum commune

  • Received:2011-12-06 Revised:2012-03-30 Online:2012-08-25 Published:2012-08-25

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摘要：

摘要：以裂褶菌蛋为研究对象，用硫酸铵沉淀其蛋白，制成蛋白粗提液，研究其诱导烟草对烟草花叶病毒（TMV）的抗性，和体外钝化烟草花叶病毒（TMV）的防治效果；结果表明：当蛋白浓度达到100μg/mL时，诱导烟草抗TMV的效果较好，侵染枯斑抑制率可达74.11%±3.7%，对TMV的体外钝化作用的枯斑抑制率为70.05%±1.25%，表明裂褶菌蛋白粗提液通过诱导抗病性和对TMV的体外钝化作用达到了控制TMV的作用；裂褶菌蛋白粗提液对辣椒病病毒（CMV）具有很好控制作用，用100μg/mL的裂褶菌蛋白粗提液处理辣椒植株后，其对CMV的平均防效为66.46%。

关键词: 紫羊茅, 紫羊茅

Abstract:

To reveal the bioactivity of Schizophyllum commune, the activity determination of anti-Tobacco mosaic virus (TMV) and Cucumber mosaic virus (CMV) were studied with crude protein extracts of S. commune. The crude protein was extracted from S. commune by the ammonium sulfate precipitation method, and the activity of inducing tobacco resistance to TMV, anti-TMV in vitro and control cayenne pepper virus caused by CMV and tomato virus disease caused by TMV were studied. The activity detection results showed that when the concentration was 100 μg/mL, the inhibition ratio of inducting resistance to TMV and anti-TMV in vitro were 74.02%±3.61% and 70.05%±1.25%, respectively. The control ratio of cayenne pepper virus disease and tomato virus disease were 66.46% and 68.50%, respectively. It showed that the crude protein extracts from S. commune could keep high activity of resistance of TMV and CMV by inducing resistance and anti-activity in vitro.

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